{
  "abstract": "Glucuronidation is an important detoxification pathway that operates in balance with gastrointestinal microbial β-glucuronidase (GUS) activity, which can regenerate bioactive metabolites from their glucuronidated forms. How this host-microbe interaction shapes the distribution and pool of glucuronidated metabolites (i.e., the glucuronidome) remains poorly understood. In this study, we employed pattern-filtering data science approaches in conjunction with untargeted LC-MS/MS metabolomics to map the glucuronidome in urine, serum, and colon/fecal samples from gnotobiotic and conventional mice, and in humans. We find that microbial colonization and GUS activity compress the colonic glucuronidome and expand urinary glucuronidome diversity, revealing a compartmental redistribution of glucuronidated metabolites. Reverse metabolomics of known glucuronidated chemicals and glucuronidation pattern filtering searches in public metabolomics datasets exposed the diversity of glucuronidated metabolites in human and mouse ecosystems. In summary, we present a glucuronidation fingerprint resource that provides broader access to and analysis of the glucuronidome. Together, this work establishes a scalable analytical framework and provides mechanistic insight into how microbial activity reshapes systemic glucuronidation, with implications for drug metabolism, diet-microbe interactions, and biomarker discovery.",
  "abstract_shown": true,
  "affiliations": [
    "Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA",
    "Departments of Chemistry, Biochemistry, Microbiology and Genomics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA",
    "Department of Computer Science, University of California Riverside, Riverside, CA, USA",
    "Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA",
    "Curriculum in Toxicology & Environmental Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA",
    "Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA",
    "Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA, USA",
    "Division of Gastroenterology and Hepatology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA, USA",
    "The Center for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA",
    "Division of Gastroenterology and Hepatology and Nutrition, Department of Pediatrics, Perelman School of Medicine, Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA, USA",
    "Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, USA",
    "Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA",
    "Center for Clinical Epidemiology and Biostatistics, Division of Gastroenterology and Hepatology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA",
    "One Health Microbiome Center, Huck Life Sciences Institute, University Park, PA, USA",
    "Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA, USA. adp117@psu.edu",
    "Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA. adp117@psu.edu",
    "Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, USA. adp117@psu.edu",
    "Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA. adp117@psu.edu",
    "One Health Microbiome Center, Huck Life Sciences Institute, University Park, PA, USA. adp117@psu.edu"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 24,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Nina R. Boyle",
      "orcid": "0000-0003-0901-3775"
    },
    {
      "affs": [
        1
      ],
      "name": "Josh J. Sekela",
      "orcid": "0000-0002-5439-491X"
    },
    {
      "affs": [
        2
      ],
      "name": "Mingxun Wang",
      "orcid": "0000-0001-7647-6097"
    },
    {
      "affs": [
        3
      ],
      "name": "Helena Mannochio-Russo",
      "orcid": "0000-0002-4961-2353"
    },
    {
      "affs": [
        4
      ],
      "name": "Jeong Joo Pyo",
      "orcid": "0000-0002-7240-021X"
    },
    {
      "affs": [
        5
      ],
      "name": "Minkyu Kim",
      "orcid": "0000-0002-3322-9448"
    },
    {
      "affs": [
        5
      ],
      "name": "Shuchang Tian",
      "orcid": "0009-0008-7920-4693"
    },
    {
      "affs": [
        6
      ],
      "name": "Imhoi Koo",
      "orcid": "0000-0002-5816-0627"
    },
    {
      "affs": [
        7,
        8
      ],
      "name": "Elliot S. Friedman",
      "orcid": "0000-0003-0050-4965"
    },
    {
      "affs": [
        9,
        8
      ],
      "name": "Ceylan Tanes",
      "orcid": "0000-0002-5823-4059"
    },
    {
      "affs": [
        10
      ],
      "name": "Mallappa Anitha"
    },
    {
      "affs": [
        10,
        0
      ],
      "name": "Yuan Tian"
    },
    {
      "affs": [
        6,
        10
      ],
      "name": "Ethan W. Morgan",
      "orcid": "0000-0002-1752-8835"
    },
    {
      "affs": [
        6,
        10
      ],
      "name": "Iain A. Murray",
      "orcid": "0000-0003-1535-375X"
    },
    {
      "affs": [
        11,
        8
      ],
      "name": "Joseph P. Zackular",
      "orcid": "0000-0002-3228-3055"
    },
    {
      "affs": [
        9,
        8
      ],
      "name": "Kyle L. Bittinger",
      "orcid": "0000-0003-3472-5934"
    },
    {
      "affs": [
        12
      ],
      "name": "James D. Lewis",
      "orcid": "0000-0003-3107-3862"
    },
    {
      "affs": [
        6,
        10
      ],
      "name": "Gary H. Perdew",
      "orcid": "0000-0001-6288-8445"
    },
    {
      "affs": [
        7
      ],
      "name": "Gary D. Wu",
      "orcid": "0000-0002-1829-8795"
    },
    {
      "affs": [
        9
      ],
      "name": "Babette S. Zemel",
      "orcid": "0000-0002-6164-7348"
    },
    {
      "affs": [
        3
      ],
      "name": "Pieter C. Dorrestein",
      "orcid": "0000-0002-3003-1030"
    },
    {
      "affs": [
        5,
        0,
        13
      ],
      "name": "Jordan E. Bisanz",
      "orcid": "0000-0002-8649-1706"
    },
    {
      "affs": [
        4,
        1
      ],
      "name": "Matthew R. Redinbo",
      "orcid": "0000-0003-0814-5346"
    },
    {
      "affs": [
        14,
        15,
        16,
        17,
        18
      ],
      "name": "Andrew D. Patterson",
      "orcid": "0000-0003-2073-0070"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c0c0mn2hfqq2v.bib",
  "cited_by": 6,
  "doi": "10.1038/s41467-026-73398-1",
  "fulltext_url": null,
  "id": "c0c0mn2hfqq2v",
  "issns": [
    "2041-1723"
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  "landing_url": "https://doi.org/10.1038/s41467-026-73398-1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
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  "pdf_url": "https://www.nature.com/articles/s41467-026-73398-1_reference.pdf",
  "pmcid": "PMC12036448",
  "pmid": "40297700",
  "published": "2026-05-20",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/c0c0mn2hfqq2v.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:48Z",
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      "url": "https://doi.org/10.1038/s41467-026-73398-1"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Metabolomics and Mass Spectrometry Studies",
      "subfield": "molecular-biology"
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  ],
  "title": "Glucuronidation metabolomic fingerprinting to map host-microbe metabolism",
  "title_text": "Glucuronidation metabolomic fingerprinting to map host-microbe metabolism",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T13:20:30Z",
  "url": "https://research.draconitic.com/paper/c0c0mn2hfqq2v",
  "venue": "Nature Communications",
  "versions": []
}