{
  "abstract": "Lentivirus (LV)-based manipulation of immune cells is a commonly used approach in cell therapies. However, the most frequently used Vesicular Stomatitis Virus Glycoprotein (VSV-G) pseudotyping approach is inefficient to support LV gene delivery of mouse immune cells due to the lack of an optimal entry receptor. Here, we present a transgenic mouse model with Cre recombinase-inducible expression of the human Low Density Lipoprotein Receptor (hLDLR), the natural entry receptor of VSV-G LV. Surprisingly, we found that Cre induces robust hLDLR expression across multiple immune cells only under activation conditions. Induction of hLDLR expression by genetic crossing with Cre-transgenic mice or exposure to Cre mRNA-lipid nanoparticles (LNPs) potently sensitized murine immune cells, especially T cells, to VSV-G LV-mediated gene delivery. This novel transgenic mouse model serves as a valuable tool for investigating immune cell function and engineered cell therapies for cancer in syngeneic mouse models.",
  "abstract_shown": true,
  "affiliations": [
    "The Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Children’s Hospital of Philadelphia",
    "Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania",
    "Department of Experimental Pathology, Children’s Hospital of Philadelphia",
    "Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania",
    "Institute of RNA Innovation, University of Pennsylvania",
    "Center for Cellular Immunotherapies, University of Pennsylvania",
    "Abramson Cancer Center, University of Pennsylvania"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jian Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Beatriz Goncalves"
    },
    {
      "affs": [
        0
      ],
      "name": "Gabrielle Melamed"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Dillon O’Neill"
    },
    {
      "affs": [
        0
      ],
      "name": "Ryan Cozzone"
    },
    {
      "affs": [
        0
      ],
      "name": "Sara Iuliucci"
    },
    {
      "affs": [
        0
      ],
      "name": "Austin Cozzone"
    },
    {
      "affs": [
        0,
        2,
        3,
        4
      ],
      "name": "Mohamad-Gabriel Alameh"
    },
    {
      "affs": [
        0,
        2,
        3,
        5,
        6
      ],
      "name": "Leyuan Ma"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c1ny3yyrx4kpv.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1941021",
  "fulltext_url": "https://europepmc.org/articles/PMC13683734",
  "id": "c1ny3yyrx4kpv",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1941021/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Virus-based gene therapy research"
    ]
  ],
  "openalex": "W7220832523",
  "pdf_url": "https://europepmc.org/articles/PMC13683734?pdf=render",
  "pmcid": "PMC13683734",
  "pmid": "42909043",
  "published": "2026-10-07",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/c1ny3yyrx4kpv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:15Z",
      "native_id": "10.3389/fimmu.2026.1941021",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1941021/full"
    },
    {
      "first_seen": "2026-10-30T12:20:14Z",
      "native_id": "MED:42909043",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42909043"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Virus-based gene therapy research",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "An inducible human LDLR transgenic mouse model for enhanced lentiviral gene delivery to murine immune cells",
  "title_text": "An inducible human LDLR transgenic mouse model for enhanced lentiviral gene delivery to murine immune cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:14Z",
  "url": "https://research.draconitic.com/paper/c1ny3yyrx4kpv",
  "venue": "Frontiers in Immunology",
  "versions": []
}