{
  "abstract": "Following the discovery of the piperazic acid (Piz)-containing hexapeptide demiguisin A from a toxic bird-associated Amycolatopsis strain, we aimed to investigate the structural diversity and bioactive potential of this natural product family. Here, we used the dmg biosynthetic gene cluster from Amycolatopsis sp. PS_44_ISF1 as a pathway mining probe to search for alternative demiguisin A producers. A global search identified only one other soil-derived Amycolatopsis sp. NBC_01488, encoding a highly syntenic pathway. Under feather-mimicking conditions, PS_44_ISF1 demonstrated enhanced growth and metabolite production relative to NBC_01488. Despite phylogenomic distance, both strains produced shared metabolites, while NBC_01488 yielded more abundant demiguisins, enabling the isolation of five novel derivatives characterized by two Piz-moieties with different hydroxylation patterns and glycine truncation. Two congeners displayed potent activity against the avian pathogen Mycobacterium intracellulare and moderate activity against Mycobacterium tuberculosis . These findings show that targeted pathway mining guided the expansion of the chemical diversity of Piz-modifications and demiguisin congeners with promising anti- Mycobacterium activity.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Tanya Decker"
    },
    {
      "name": "Marie Dayras",
      "orcid": "0000-0002-2598-7943"
    },
    {
      "name": "Lena Schweizer",
      "orcid": "0000-0003-1296-5486"
    },
    {
      "name": "Sari Rasheed",
      "orcid": "0000-0002-9700-0474"
    },
    {
      "name": "Franziska Fries",
      "orcid": "0009-0009-6485-8317"
    },
    {
      "name": "Bulisa Iova"
    },
    {
      "name": "Kasun H. Bodawatta",
      "orcid": "0000-0002-6095-9059"
    },
    {
      "name": "Knud Andreas Jønsson",
      "orcid": "0000-0002-1875-9504"
    },
    {
      "name": "Rolf Müller",
      "orcid": "0000-0002-1042-5665"
    },
    {
      "name": "Christine Beemelmanns",
      "orcid": "0000-0002-9747-3423"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c34wnne2cpzz9.bib",
  "cited_by": 0,
  "doi": "10.1038/s42004-026-02250-w",
  "fulltext_url": "https://europepmc.org/articles/PMC13676617",
  "id": "c34wnne2cpzz9",
  "issns": [
    "2399-3669"
  ],
  "landing_url": "https://www.nature.com/articles/s42004-026-02250-w",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2736|Microbial Natural Products and Biosynthesis"
    ]
  ],
  "openalex": "W7222801642",
  "pdf_url": "https://www.nature.com/articles/s42004-026-02250-w.pdf",
  "pmcid": "PMC13676617",
  "pmid": "42892638",
  "published": "2026-10-17",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/c34wnne2cpzz9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:51Z",
      "native_id": "10.1038/s42004-026-02250-w",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s42004-026-02250-w"
    },
    {
      "first_seen": "2026-10-30T12:18:46Z",
      "native_id": "MED:42892638",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42892638"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Microbial Natural Products and Biosynthesis",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Targeted pathway mining reveals novel modified hexapeptides from the demiguisin family",
  "title_text": "Targeted pathway mining reveals novel modified hexapeptides from the demiguisin family",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:46Z",
  "url": "https://research.draconitic.com/paper/c34wnne2cpzz9",
  "venue": "Communications Chemistry",
  "versions": []
}