{
  "abstract": "Bacterial biofilms drive persistent infection and chronic inflammation, yet host sensing of biofilm-derived ligands remains poorly understood. Here we identify curli amyloid, an integral component of Enterobacteriaceae biofilms, as a novel activator of the non-canonical inflammasome in human and mouse dendritic cells (DCs). Curli both primes and activates the inflammasome, directly binding caspase-11 to engage NLRP3 and drive robust IL-1{beta} maturation without pyroptosis, establishing curli as a previously unrecognized trigger of DC hyperactivation. Mechanistically, this non-lytic signaling is marked by limited gasdermin D oligomerization without mitochondrial localization and NINJ1-dependent membrane rupture. Functionally, curli-hyperactivated DCs show enhanced migration to the draining lymph nodes in vivo, suggesting consequent augmented adaptive immunity. Finally, curli-containing biofilms also elicit IL-1{beta} secretion without cell death, identifying bacterial amyloid as a central driver of non-lytic inflammasome activation during immune recognition of biofilms.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Dendritic Cell Biology, Division of Innate Immunity, Department of Medicine, University of Massachusetts Chan Medical School, Worcester, MA, 01545"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "S. Mintri"
    },
    {
      "name": "G. Hu"
    },
    {
      "name": "A. Mathur"
    },
    {
      "name": "L. M. Whiteman"
    },
    {
      "name": "A. Dinis"
    },
    {
      "name": "B. Le"
    },
    {
      "name": "K. Grando"
    },
    {
      "name": "P. Shumba"
    },
    {
      "name": "V. A. Rathinam"
    },
    {
      "name": "E. Lien"
    },
    {
      "name": "C. Tukel"
    },
    {
      "name": "K. A. Fitzgerald"
    },
    {
      "affs": [
        0
      ],
      "name": "S. Gallucci"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c38ebjk5130eh.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.29.755159",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755159v1.full",
  "id": "c38ebjk5130eh",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755159v1",
  "language": null,
  "licence": "CC-BY-ND",
  "licence_label": "CC BY-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "immunology"
    ],
    [
      "openalex",
      "1312|Inflammasome and immune disorders"
    ]
  ],
  "openalex": "W7219696439",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755159v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/c38ebjk5130eh.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:25:57Z",
      "native_id": "10.64898/2026.09.29.755159",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755159v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:immunology",
      "subfield": "immunology"
    }
  ],
  "title": "A Novel Biofilm Amyloid Ligand for Caspase-11 Drives Hyperactivation in Dendritic Cells",
  "title_text": "A Novel Biofilm Amyloid Ligand for Caspase-11 Drives Hyperactivation in Dendritic Cells",
  "type": "preprint",
  "updated_at": "2026-10-30T12:25:57Z",
  "url": "https://research.draconitic.com/paper/c38ebjk5130eh",
  "venue": "bioRxiv",
  "versions": []
}