{
  "abstract": "Neuropathic pain (NP) is linked to disrupted sleep and circadian abnormalities upon painful peripheral nerve injury. By performing spared nerve injury (SNI) in circadian reporter PER2::LUC mice, we characterized the real-time changes in rhythmicity of the core clock gene Per2 in the pain-regulating dorsal root ganglia (DRG), and the sleep-wake cycle using wireless EEG/EMG under NP conditions. We further evaluated whether the clock-resynchronizing flavonoids, nobiletin (2 mg/kg/day) and tangeretin (1.8 mg/kg/day), administered continuously via osmotic minipumps for two weeks, could restore circadian dynamics and alleviate pain-related behaviour. Both flavonoids alleviated SNI-induced dynamic hypersensitivity in PER2::LUC mice on post-surgical day 14. SNI increased the amplitude of Per2 rhythms in the DRGs, which was restored to pre-surgical levels by administration of flavonoids. In addition, tangeretin showed sleep-restoring properties upon SNI by alleviating disturbances in NREM sleep and wakefulness. These results support targeting circadian disruption as a potential chronotherapeutic approach to alleviate NP.",
  "abstract_shown": true,
  "affiliations": [
    "University of Helsinki"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "H. Launonen"
    },
    {
      "name": "W. Dai"
    },
    {
      "name": "M. Wen"
    },
    {
      "name": "N. Milicevic"
    },
    {
      "name": "M.-K. Koskinen"
    },
    {
      "name": "A. Pertovaara"
    },
    {
      "name": "E. Kalso"
    },
    {
      "name": "H.-K. Wigren"
    },
    {
      "affs": [
        0
      ],
      "name": "V. Palada"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c3qah7gm6xn2s.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.26.761906",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761906v1.full",
  "id": "c3qah7gm6xn2s",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761906v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "neuroscience"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761906v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-26",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/c3qah7gm6xn2s.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:10Z",
      "native_id": "10.64898/2026.10.26.761906",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761906v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:neuroscience",
      "subfield": "neuroscience"
    }
  ],
  "title": "Flavonoid chronotherapy normalizes circadian dynamics and sleep architecture in mice with painful nerve injury",
  "title_text": "Flavonoid chronotherapy normalizes circadian dynamics and sleep architecture in mice with painful nerve injury",
  "type": "preprint",
  "updated_at": "2026-11-01T00:21:40Z",
  "url": "https://research.draconitic.com/paper/c3qah7gm6xn2s",
  "venue": "bioRxiv",
  "versions": []
}