{
  "abstract": "Focal adhesion kinase (FAK) is a protein tyrosine kinase that plays a major role in regulating cell adhesion, proliferation, motility, and survival. While FAK-regulatory phosphosites play significant roles in cancer progression and metastasis, the broader perspective of its phosphorylation remains largely unknown. Therefore, mapping the phosphoregulatory networks provides significant insights into targeted therapies. To characterize the phosphorylations of FAK, we systematically retrieved PubMed-indexed studies and compiled large-scale cellular mass spectrometry-based phosphoproteomics datasets. An integrative, data-driven analysis was performed on 678 qualitative and 235 quantitative cellular phosphoproteomic datasets to map the signatures of FAK phosphorylation. The study observed that the S910 modification is the most frequently detected phosphorylation outside the catalytic kinase domain. This predicts a broader regulatory role of S910 in signaling than previously understood. Further, by integrating phosphosite co-detection patterns across diverse biological contexts, we characterized FAK phosphorylations and their co-regulated Phosphosites in Other Proteins (PsOPs). The phospho-coregulatory analysis of S910 and FAK activation phosphosites Y576, Y577, and Y861 provided distinct potential phosphosite cross-talks. Furthermore, the study predicts several potential candidate substrates and interactor PsOPs, providing a framework for prioritizing phosphosites for future experimental investigation. Moreover, the study also illustrated a hypothesized model of potential FAK phosphosite-specific coregulatory associations within gastrin-associated signaling. Taken together, these large-scale observations provide a data-driven foundation for future FAK-centered experimental approaches and understanding of its multifaceted role as a potential target.",
  "abstract_shown": true,
  "affiliations": [
    "Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575018, Karnataka, India",
    "Centre for Integrative Omics Data Science, Yenepoya (Deemed to be University), Mangalore 575018, Karnataka, India",
    "Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia",
    "Central Research Laboratory, Srinivas University, Mukka 574146, Karnataka, India"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Mejo George"
    },
    {
      "affs": [
        1
      ],
      "name": "Leona Dcunha",
      "orcid": "0009-0004-2245-9522"
    },
    {
      "affs": [
        2
      ],
      "name": "Mukhtar Ahmed"
    },
    {
      "affs": [
        1
      ],
      "name": "Althaf Mahin",
      "orcid": "0009-0007-6017-4415"
    },
    {
      "affs": [
        1
      ],
      "name": "Athira Perunelly Gopalakrishnan"
    },
    {
      "affs": [
        0
      ],
      "name": "Prashant Kumar Modi",
      "orcid": "0000-0002-4817-3379"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Anoop Kumar G. Velikkakath"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Rajesh Raju",
      "orcid": "0000-0003-2319-121X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c47t4tq39f0vb.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219484",
  "fulltext_url": null,
  "id": "c47t4tq39f0vb",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9484",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2723|Cell Adhesion Molecules Research"
    ]
  ],
  "openalex": "W7223826206",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-23",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/c47t4tq39f0vb.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:57Z",
      "native_id": "10.3390/ijms27219484",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9484"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2723|Cell Adhesion Molecules Research",
      "subfield": "immunology"
    }
  ],
  "title": "An Integrative Phosphoregulatory Landscape of FAK Signaling",
  "title_text": "An Integrative Phosphoregulatory Landscape of FAK Signaling",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:57Z",
  "url": "https://research.draconitic.com/paper/c47t4tq39f0vb",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}