{
  "abstract": "Ferroptosis contributes to ultraviolet (UV)-induced epidermal injury, but whether circadian state modifies keratinocyte susceptibility remains unclear. We integrated human epidermal transcriptomic analyses with functional experiments in HaCaT keratinocytes to compare UVA- and UVB-induced ferroptosis-associated responses and assess their temporal regulation. Both wavelengths reduced cell viability and increased reactive oxygen species, lipid peroxidation, and intracellular Fe2+ accumulation; ferrostatin-1 provided partial protection. UVB reduced xCT and GPX4 and showed a proteasome-sensitive component of GPX4 loss, whereas UVA predominantly reduced GPX4 without detectably altering xCT. Human epidermal transcriptomes showed heterogeneous cross-sectional associations among circadian, oxidative-stress, and ferroptosis-related genes, while longitudinal profiles revealed positive temporal coupling between NR1D1-associated signals and GPX4. In synchronized HaCaT cells, ARNTL and NR1D1 displayed 24 h rhythmicity, whereas GPX4 and SLC7A11 did not meet the prespecified rhythmicity criterion. Nevertheless, UVA-induced cytotoxicity, oxidative stress, lipid peroxidation, and GPX4 loss varied across post-synchronization time points and were greatest at 24 h. SR8278 partially preserved GPX4 abundance and attenuated UVA-induced loss of viability, reactive oxygen species accumulation, and lipid peroxidation, without significantly affecting Fe2+ accumulation. These findings associate circadian state with temporal variation in UVA-induced ferroptosis susceptibility and implicate the broader clock-redox system rather than rhythmic expression of individual ferroptosis effectors.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Molecular Epidemiology of Hunan Province, School of Public Health, Health Science Center, Hunan Normal University, Changsha 410013, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ren Dong"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiang Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Ping Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Qianxian Yin"
    },
    {
      "affs": [
        0
      ],
      "name": "Zirun Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Ying Xie",
      "orcid": "0000-0001-7568-2569"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c4tcb9wp3n18k.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27208947",
  "fulltext_url": null,
  "id": "c4tcb9wp3n18k",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/8947",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2740|Ferroptosis and cancer prognosis"
    ]
  ],
  "openalex": "W7221130576",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-09",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/c4tcb9wp3n18k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27208947",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/8947"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2740|Ferroptosis and cancer prognosis",
      "subfield": "general"
    }
  ],
  "title": "Temporal Regulation of UVA-Induced Ferroptosis-Associated Injury in Keratinocytes",
  "title_text": "Temporal Regulation of UVA-Induced Ferroptosis-Associated Injury in Keratinocytes",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:55Z",
  "url": "https://research.draconitic.com/paper/c4tcb9wp3n18k",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}