{
  "abstract": "Engineered bacteria are being explored as oral delivery systems for therapeutic peptides. This study examined Lactobacillus acidophilus expressing two GLP-1 peptide variants with N-terminal extensions, M-GLP-1 and M-6HIS-GLP-1, in male C57BL/6J mice with moderate streptozotocin (STZ)-induced hyperglycemia. Both purified peptides increased insulin concentrations in MIN6 conditioned medium after 24 h, but neither significantly attenuated the STZ-associated reduction in metabolic activity. Cage-level analysis showed lower average post-treatment glucose concentrations in mice receiving L. acidophilus expressing M-GLP-1 (Lacto-M-GLP-1) or M-6HIS-GLP-1 (Lacto-M-6HIS-GLP-1) than in STZ controls, but neither group differed significantly from mice receiving empty-vector L. acidophilus. The intraperitoneal glucose tolerance test showed numerically lower late-phase glucose concentrations and incremental area under the curve in both groups than in controls. Mice receiving Lacto-M-6HIS-GLP-1 showed greater body-weight change than those receiving Lacto-M-GLP-1 or empty-vector L. acidophilus. Pancreatic histology showed a lower pancreatic glucagon-positive signal area and a higher insulin-to-glucagon signal-area ratio than in STZ controls and Lacto-M-GLP-1. The findings demonstrate cellular responses to purified GLP-1 variants and metabolic changes associated with administering L. acidophilus expressing the peptides.",
  "abstract_shown": true,
  "affiliations": [
    "Koltzov Institute of Developmental Biology of the Russian Academy of Sciences, 119334 Moscow, Russia"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Arthur A. Lee",
      "orcid": "0009-0001-3341-4248"
    },
    {
      "affs": [
        0
      ],
      "name": "Maria B. Chernysheva",
      "orcid": "0000-0001-5248-5028"
    },
    {
      "affs": [
        0
      ],
      "name": "Dmitry A. Kashirskikh",
      "orcid": "0000-0002-0748-9238"
    },
    {
      "affs": [
        0
      ],
      "name": "Evgenii S. Ruchko",
      "orcid": "0000-0002-1361-666X"
    },
    {
      "affs": [
        0
      ],
      "name": "Olesya O. Klychkova",
      "orcid": "0009-0000-1308-4942"
    },
    {
      "affs": [
        0
      ],
      "name": "Victoria A. Khotina",
      "orcid": "0000-0003-2096-3237"
    },
    {
      "affs": [
        0
      ],
      "name": "Tatiana V. Noskova"
    },
    {
      "affs": [
        0
      ],
      "name": "Vagif A. Gasanov"
    },
    {
      "affs": [
        0
      ],
      "name": "Ekaterina A. Vorotelyak",
      "orcid": "0000-0001-5405-0212"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrey V. Vasiliev"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c65y8kf4v0aya.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms272110003",
  "fulltext_url": null,
  "id": "c65y8kf4v0aya",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/10003",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-08",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/c65y8kf4v0aya.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:22:39Z",
      "native_id": "10.3390/ijms272110003",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/10003"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "Oral Administration of Lactobacillus acidophilus Expressing GLP-1 Peptide Variants with N-Terminal Extensions in Mice with Streptozotocin-Induced Hyperglycemia",
  "title_text": "Oral Administration of Lactobacillus acidophilus Expressing GLP-1 Peptide Variants with N-Terminal Extensions in Mice with Streptozotocin-Induced Hyperglycemia",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:22:39Z",
  "url": "https://research.draconitic.com/paper/c65y8kf4v0aya",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}