{
  "abstract": "MicroRNAs are essential for worm development, yet their roles remain unclear in most tissues. Here, we established a tissue-specific Argonaute knockout system in Caenorhabditis elegans using conditional deletion of alg-1 in an alg-2 mutant background. Tissue-restricted loss of Argonaute revealed distinct developmental and tissue-specific phenotypes. Argonaute depletion in the intestine, pharynx, and hypodermis caused developmental arrest and tissue-specific defects, whereas neuronal and muscle-specific Argonaute depletion impaired locomotion and affected neuronal or muscle-associated phenotypes. Moreover, combined loss of selected tissue-expressed microRNAs only partially recapitulated the corresponding Argonaute-depletion phenotypes. Muscle-specific re-expression of selected microRNAs rescued the locomotion defect of the compound microRNA mutant. This study establishes a platform for dissecting tissue-specific microRNA functions during animal development.",
  "abstract_shown": true,
  "affiliations": [
    "College of Life Sciences, Capital Normal University , Beijing 100048 ,",
    "School of Life Sciences, Tsinghua University , Beijing 100084 ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Yimeng Gao"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuchuan Bai"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaokun Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yiru Jiang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yongbin Li",
      "orcid": "0000-0002-0686-0883"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c6kkhynywtzff.bib",
  "cited_by": 0,
  "doi": "10.1093/g3journal/jkag279",
  "fulltext_url": null,
  "id": "c6kkhynywtzff",
  "issns": [
    "2160-1836"
  ],
  "landing_url": "https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag279/8851441",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1306|MicroRNA in disease regulation"
    ]
  ],
  "openalex": "W7214900070",
  "pdf_url": "https://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkag279/71499285/jkag279.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-30",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/c6kkhynywtzff.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:09Z",
      "native_id": "10.1093/g3journal/jkag279",
      "source": "crossref",
      "url": "https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag279/8851441"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|MicroRNA in disease regulation",
      "subfield": "oncology"
    }
  ],
  "title": "Conditional Argonaute knockout reveals tissue-specific requirements of microRNA activity in C. elegans",
  "title_text": "Conditional Argonaute knockout reveals tissue-specific requirements of microRNA activity in C. elegans",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:09Z",
  "url": "https://research.draconitic.com/paper/c6kkhynywtzff",
  "venue": "G3: Genes, Genomes, Genetics",
  "versions": []
}