{
  "abstract": "Broad-spectrum phage resistance offers an important layer of protection against bacterial fermenter crashes during biomanufacturing processes, yet the underlying mechanisms are often poorly defined or come at a fitness cost. Using experimental evolution, we generated two Pseudomonas putida strains that were resistant to at least six phage genera. Genome sequencing revealed a single frameshift deletion in each strain that restored functionality of a Type I secretion system (T1SS) ATPase. We also found strong transcriptional upregulation of a nearby protein, PP_1794, which we coin Phage shielding helix rich protein (Psh). Overexpression experiments show that Psh protein is secreted by this restored T1SS to confer complete phage resistance. When compared to wild-type P. putida KT2440, no growth or expression defects were evident, but pyoverdine production was reduced. The Psh gene and T1SS are widely distributed across Gram-negative bacteria. These findings uncover a previously uncharacterized phage defense system in P. putida based on secretion-mediated receptor masking.",
  "abstract_shown": true,
  "affiliations": [
    "Sandia National Laboratories"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "B. Diaz"
    },
    {
      "name": "S. Won"
    },
    {
      "name": "J. D. Jaryenneh"
    },
    {
      "name": "M. M. Rahman"
    },
    {
      "name": "V. R. Chourasia"
    },
    {
      "affs": [
        0
      ],
      "name": "C. M. Mageeney"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c70bj9s4ktqph.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.02.748959",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.02.748959v1.full",
  "id": "c70bj9s4ktqph",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.02.748959v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "2303|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7208770552",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.02.748959v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-02",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/c70bj9s4ktqph.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.64898/2026.09.02.748959",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.02.748959v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Phage proofing Pseudomonas putida uncovers novel broad-spectrum phage resistance protein Psh",
  "title_text": "Phage proofing Pseudomonas putida uncovers novel broad-spectrum phage resistance protein Psh",
  "type": "preprint",
  "updated_at": "2026-10-30T12:19:12Z",
  "url": "https://research.draconitic.com/paper/c70bj9s4ktqph",
  "venue": "bioRxiv",
  "versions": []
}