{
  "abstract": "Inflammatory signaling in the human myometrium drives uterine activation and labor, yet its transcriptional regulation remains incompletely understood. We previously identified MAFF as an inflammation-inducible transcription factor in human myometrial cells. Here, we investigated whether FOS regulates MAFF expression and whether MAFF contributes to myometrial cell contractility. FOS knockdown increased MAFF mRNA approximately 1.5-fold under basal conditions and 1.7-fold following IL1B stimulation, accompanied by increased MAFF protein after 1 h of IL1B exposure. ChIP-qPCR demonstrated FOS occupancy in the MAFF promoter region, supporting a negative regulatory relationship. Functionally, CRISPR/Cas9-generated MAFF-deficient cells displayed approximately 15–25% lower basal contractility than wild-type cells. Contractile responses to IL1B varied among the MAFF-deficient clones, although they generally remained less contractile than wild-type cells. MAFF overexpression increased contraction in wildtype cells (by 7%) and two of three MAFF-deficient clones (by 4–8%). Conversely, FOS overexpression reduced contraction in wild-type cell (−6.7%) but had no significant effect in the MAFF-deficient clones. Together, these findings identify FOS as a negative regulator of MAFF expression and support a role for MAFF in modulating myometrial cell contractility.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "Eduardo Orozco-Alonso",
      "orcid": "0000-0003-4789-5182"
    },
    {
      "name": "Palak Gujral",
      "orcid": "0009-0002-1377-8615"
    },
    {
      "name": "James Saliba",
      "orcid": "0000-0003-0450-9616"
    },
    {
      "name": "Volker Blank",
      "orcid": "0000-0002-7029-2462"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c7egje6rveynk.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-73274-4",
  "fulltext_url": null,
  "id": "c7egje6rveynk",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-73274-4",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Preterm Birth and Chorioamnionitis"
    ]
  ],
  "openalex": "W7218806444",
  "pdf_url": "https://www.nature.com/articles/s41598-026-73274-4_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-03",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/c7egje6rveynk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:19Z",
      "native_id": "10.1038/s41598-026-73274-4",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-73274-4"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Preterm Birth and Chorioamnionitis",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "The FOS transcription factor negatively regulates MAFF to control myometrial cell contractility",
  "title_text": "The FOS transcription factor negatively regulates MAFF to control myometrial cell contractility",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:19Z",
  "url": "https://research.draconitic.com/paper/c7egje6rveynk",
  "venue": "Scientific Reports",
  "versions": []
}