{
  "abstract": "Leishmania CK1.2 (L-CK1.2) is a serine/threonine protein kinase essential for the survival of the protozoan parasite Leishmania, the causative agent of leishmaniasis. This study investigates the dynamic localization pattern of L-CK1.2 and the broad spectrum of its interacting partners within the parasite. Using proteomic analysis and confocal microscopy, we identified 230 L-CK1.2-associated proteins across the major parasite life stages. This analysis revealed the ubiquitous presence of L-CK1.2 in various cellular structures, including the cytoskeleton, basal body, and flagellum. Using an in vitro system, sixty-four host proteins, involved in critical host biological processes such as immune response, apoptosis, and purine biosynthesis, were identified as potential interacting partners of L-CK1.2. These processes are known to be regulated by Leishmania during infection. The study highlights the potential dual function of L-CK1.2, in the parasite (cis) and within the host cell (trans), positioning this kinase as a key player in host-pathogen interactions. This work provides a comprehensive map of L-CK1.2 interactions and suggests its potential importance in regulating intracellular Leishmania survival, providing potential targets for leishmaniasis therapy. Furthermore, given the evolutionary conservation of CK1.2 across other parasitic organisms, our findings may have broader implications for understanding and managing parasitic infections.",
  "abstract_shown": true,
  "affiliations": [
    "Institut Pasteur, Université Paris Cité and INSERM U1347, Groupe de signalisation et interactions hôte-parasite, Paris, France",
    "Institut Pasteur, Université Paris Cité and INSERM U1347, Unité de Parasitologie moléculaire et Signalisation, Paris, France",
    "Laboratoire de Spectrométrie de Masse Protéomique (LSMP), Centre de Recherche, Institut Curie, PSL Research University, Paris, France"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Daniel Martel"
    },
    {
      "affs": [
        1
      ],
      "name": "Olivier Leclercq"
    },
    {
      "affs": [
        2
      ],
      "name": "Florent Dingli"
    },
    {
      "name": "Victor Laigle"
    },
    {
      "name": "Nawal Hajj Sleiman"
    },
    {
      "name": "Damarys Loew"
    },
    {
      "name": "Gerald F. Späth"
    },
    {
      "name": "Najma Rachidi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c7kh1sh9szsma.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0356818",
  "fulltext_url": null,
  "id": "c7kh1sh9szsma",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0356818",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "openalex",
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      "plos",
      "/Biology and life sciences/Cell biology/Cellular structures and organelles/Flagella"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular types/Animal cells/Blood cells/White blood cells/Macrophages"
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    ],
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      "/Biology and life sciences/Developmental biology/Life cycles/Protozoan life cycles/Amastigotes"
    ],
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      "/Biology and life sciences/Developmental biology/Life cycles/Protozoan life cycles/Promastigotes"
    ],
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      "plos",
      "/Biology and life sciences/Genetics/Genomics/Genome analysis/Gene ontologies"
    ],
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      "plos",
      "/Biology and life sciences/Immunology/Immune cells/White blood cells/Macrophages"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Protozoology/Protozoan life cycles/Amastigotes"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Protozoology/Protozoan life cycles/Promastigotes"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Eukaryota/Protozoans/Parasitic protozoans/Leishmania"
    ],
    [
      "plos",
      "/Medicine and health sciences/Immunology/Immune cells/White blood cells/Macrophages"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogenesis/Host-pathogen interactions"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Virulence factors/Pathogen motility/Flagella"
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  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0356818&type=printable",
  "pmcid": null,
  "pmid": "42832601",
  "published": "2026-10-05",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/c7kh1sh9szsma.ris",
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    {
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      "native_id": "10.1371/journal.pone.0356818",
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      "first_seen": "2026-11-06T12:20:44Z",
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      "url": "https://hal.science/hal-05854390v1"
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  "subjects": [
    {
      "field": "biology",
      "origin": "plos:cell biology",
      "subfield": "cell-biology"
    },
    {
      "field": "biology",
      "origin": "plos:developmental biology",
      "subfield": "developmental-biology"
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    {
      "field": "biology",
      "origin": "plos:immunology",
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    {
      "field": "biology",
      "origin": "hal:1.sdv.mp",
      "subfield": "microbiology"
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  "title": "Characterization of the dual functions of Leishmania CK1.2 in both the parasite and the macrophage",
  "title_text": "Characterization of the dual functions of Leishmania CK1.2 in both the parasite and the macrophage",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:20:49Z",
  "url": "https://research.draconitic.com/paper/c7kh1sh9szsma",
  "venue": "PLOS One",
  "versions": []
}