{
  "abstract": "Ongoing research investigates the use of bromoform and other brominated halomethanes as antimethanogenic additives for ruminant livestock. Recently, iodoform has also been shown to be a potent methane inhibitor in dairy cows. To evaluate potential associated effects of using iodoform as a feed additive, cell viability assays were conducted using 2 cell lines: bovine mammary epithelial cells (MAC-T) and human intestinal epithelial cells (FHs 74 Int.), representing mammary and intestinal epithelial cell models, respectively. Cells were cultivated in media containing 1%, 5%, or 10% (vol/vol) of milk whey or blood serum sampled from multicannulated (ruminal, duodenal, and ileal) Danish Holstein dairy cows that were dosed intraruminally daily with iodoform (0, 320, 640, and 800 mg/d). The iodoform dosages were determined based on a previous unpublished pilot study. Moreover, we also investigated the effects of the direct addition of 7 different concentrations (0.003–10 µM) of the pure compounds iodoform, diiodomethane, and iodomethane on the viability of MAC-T cells. Cell viability was increased in both MAC-T and FHs 74 Int. cell lines with increased concentration of serum or whey, whether from control (0 mg/d) or iodoform-supplemented cows. No significant linear or quadratic effects of serum or whey were observed on either cell line. Consistent with these findings, MAC-T cell viability was unaffected by exposure to iodoform or its reduced form, iodomethane, across concentrations of 0.003 to 7 µM. In contrast, diiodomethane reduced cell viability at concentrations >0.5 µM; however, these levels exceed those expected in vivo following iodoform supplementation. Overall, intraruminal administration of iodoform to dairy cows was not associated with adverse effects of serum or whey on MAC-T or FHs 74 Int. cell viability.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Animal and Veterinary Sciences, Research Centre Foulum, Aarhus University Viborg, DK-8830 Tjele, Denmark"
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Marie Rønn"
    },
    {
      "affs": [
        0
      ],
      "name": "Mirka Thorsteinsson"
    },
    {
      "affs": [
        0
      ],
      "name": "Mette Olaf Nielsen"
    },
    {
      "affs": [
        0
      ],
      "name": "Stig Purup"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c81ncjb7ad170.bib",
  "cited_by": 0,
  "doi": "10.3168/jdsc.2025-1048",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13751233/?tool=EBI",
  "id": "c81ncjb7ad170",
  "issns": [
    "2666-9102"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13751233",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Physiology"
    ],
    [
      "heading",
      "Short Communication"
    ],
    [
      "openalex",
      "1103|Ruminant Nutrition and Digestive Physiology"
    ]
  ],
  "openalex": "W7196945020",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13751233/pdf/?tool=EBI",
  "pmcid": "PMC13751233",
  "pmid": "43016495",
  "published": "2026-08-06",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/c81ncjb7ad170.ris",
  "sources": [
    {
      "first_seen": "2026-11-12T15:23:59Z",
      "native_id": "PMC:PMC13751233",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13751233"
    },
    {
      "first_seen": "2026-11-14T12:23:43Z",
      "native_id": "PMC13751233",
      "source": "pmc",
      "url": "https://doi.org/10.3168/jdsc.2025-1048"
    },
    {
      "first_seen": "2026-11-14T15:20:46Z",
      "native_id": "MED:43016495",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/43016495"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "heading:Physiology",
      "subfield": "physiology"
    }
  ],
  "title": "Safety aspects of using iodoform as a methane-mitigating feed additive to dairy cows: Impacts of milk and serum on bovine mammary and human intestinal epithelial cell lines",
  "title_text": "Safety aspects of using iodoform as a methane-mitigating feed additive to dairy cows: Impacts of milk and serum on bovine mammary and human intestinal epithelial cell lines",
  "type": "peer-reviewed",
  "updated_at": "2026-11-15T12:20:50Z",
  "url": "https://research.draconitic.com/paper/c81ncjb7ad170",
  "venue": "JDS communications",
  "versions": []
}