{
  "abstract": "callus culture medium (RSC-EXO) and evaluated their molecular features and biological activities in skin-related cellular models. Nanoparticle tracking analysis and cryo-electron microscopy showed that RSC-EXO exhibited a nanoscale size distribution and spherical morphology. Western blotting confirmed enrichment of the plant EV-associated markers PEN1 and TET8. RSC-EXO were efficiently internalized by human dermal fibroblasts and showed markedly improved biocompatibility compared with crude conditioned medium (RSC-CM). Functionally, RSC-EXO significantly increased collagen synthesis and showed a trend toward enhanced wound closure in fibroblasts. In addition, RSC-EXO reduced melanin production in α-MSH-stimulated B16F10 melanoma cells and suppressed the secretion of pro-inflammatory cytokines, including IL-1α, IL-6, and TNF-α, in LPS-stimulated RAW 264.7 macrophages. Proteomic analysis revealed a distinct cargo enriched in stress-, defense-, and metabolism-related proteins, while small RNA sequencing identified a heterogeneous small RNA population containing a limited fraction of miRNA-sized reads. Collectively, these findings suggest that RSC-EXO represents a biologically active plant-derived vesicle population with regenerative and anti-inflammatory activity observed in vitro in skin-related cellular models and support its potential as a promising platform for future cosmeceutical and dermatological applications.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Prescriptionology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; jjl1217@khu.ac.kr (J.J.L.); ebmclinic@khu.ac.kr (J.Y.H.); comskp@khu.ac.kr (S.K.P.); chpark76@khu.ac.kr (C.P.); mschang@khu.ac.kr (M.S.C.)",
    "ExoCoBio Exosome Institute (EEI), ExoCoBio Inc., Seoul 08594, Republic of Korea; ceo@exocobio.com (B.S.C.); hyunju.lee@exocobio.com (H.J.L.); bogeun.son@exocobio.com (B.S.); esther.lee@exocobio.com (E.L.); sangyun.moon@exocobio.com (S.Y.M.); ella.shin@exocobio.com (E.S.); dh.ha@exocobio.com (D.H.H.)",
    "Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea; Natural Science Institute, Hanyang University, Seoul 04763, Republic of Korea; Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea; kyungminlee@han-yang.ac.kr (K.L.)"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Byong Seung Cho",
      "orcid": "0000-0002-5666-2666"
    },
    {
      "affs": [
        1
      ],
      "name": "Hyun Jung Lee"
    },
    {
      "affs": [
        1
      ],
      "name": "Bogeun Son"
    },
    {
      "affs": [
        1
      ],
      "name": "Esther Lee"
    },
    {
      "affs": [
        1
      ],
      "name": "Sang Yun Moon"
    },
    {
      "affs": [
        1
      ],
      "name": "Ella Shin"
    },
    {
      "affs": [
        0
      ],
      "name": "Jeong Jin Lee"
    },
    {
      "affs": [
        0
      ],
      "name": "Jun Young Hur"
    },
    {
      "affs": [
        0
      ],
      "name": "Seong Kyu Park",
      "orcid": "0000-0002-3589-6180"
    },
    {
      "affs": [
        0
      ],
      "name": "Cholhyun Park"
    },
    {
      "affs": [
        2
      ],
      "name": "Kyung-Min Lee",
      "orcid": "0000-0001-8381-2423"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Dae Hyun Ha",
      "orcid": "0000-0003-4508-4212"
    },
    {
      "affs": [
        0
      ],
      "name": "Mun Seog Chang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c9dqpbvtv9xrw.bib",
  "cited_by": 27,
  "doi": "10.3390/ijms27114938",
  "fulltext_url": null,
  "id": "c9dqpbvtv9xrw",
  "issns": [
    "1422-0067",
    "1661-6596"
  ],
  "landing_url": "https://doi.org/10.3390/ijms27114938",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
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  "openalex": "W7163152488",
  "pdf_url": null,
  "pmcid": "PMC13256896",
  "pmid": "42278464",
  "published": "2026-05-29",
  "publisher": "Multidisciplinary Digital Publishing Institute",
  "ris": "https://research.draconitic.com/paper/c9dqpbvtv9xrw.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:46Z",
      "native_id": "W7163152488",
      "source": "openalex",
      "url": "https://doi.org/10.3390/ijms27114938"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Characterization of Rosa damascena Callus-Derived Exosome-like Vesicles and Their Multifunctional Activities in Skin-Related Cellular Models",
  "title_text": "Characterization of Rosa damascena Callus-Derived Exosome-like Vesicles and Their Multifunctional Activities in Skin-Related Cellular Models",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T13:20:41Z",
  "url": "https://research.draconitic.com/paper/c9dqpbvtv9xrw",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}