{
  "abstract": "Macrophages, alongside mammary epithelial cells, are major contributors to the innate immune response triggered by bacterial invasion of the mammary gland. To investigate breed-specific immune mechanisms, we stimulated monocyte-derived macrophages from Holstein and Normande cattle with Escherichia coli and Streptococcus uberis, two major mastitis pathogens. We monitored bacterial survival using [methode] and profiled gene expression via RNA-seq. E. coli exhibited significantly higher survival 6 hours post-infection compared to S. uberis (p < 0.05). Moreover, E. coli induced a robust transcriptional response, with 4403 differentially expressed genes (FDR < 0.05, |log2FC| > 1), versus only 813 for S. uberis. Qualitative differences were also observed: genes such as CCL2 and CSF1 were uniquely induced by S. uberis. Additionally, macrophages from Holstein and Normande cattle showed distinct expression profiles: 185, 45, and 174 genes were differentially expressed at steady state or after E. coli or S. uberis stimulation, respectively. These genes were enriched in immune-related pathways. Overall, our results demonstrate a stronger response to E. coli, although S. uberis specifically induces a subset of genes. The observed breed-specific differences may reflect underlying immune mechanisms that could influence disease susceptibility, warranting further functional validation.",
  "abstract_shown": true,
  "affiliations": [
    "INRAE Centre Val de Loire: Institut National de Recherche pour l'Agriculture l'Alimentation et l'Environnement Centre Val de Loire"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "M. Ferter"
    },
    {
      "name": "C. Gitton"
    },
    {
      "name": "D. F. Bamu"
    },
    {
      "name": "M. Gaborit"
    },
    {
      "affs": [
        0
      ],
      "name": "P. Germon"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/c9kyxf5na7eng.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.29.755538",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755538v1.full",
  "id": "c9kyxf5na7eng",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755538v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "pathology"
    ],
    [
      "openalex",
      "1102|Milk Quality and Mastitis in Dairy Cows"
    ]
  ],
  "openalex": "W7220719418",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755538v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/c9kyxf5na7eng.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:18Z",
      "native_id": "10.64898/2026.09.29.755538",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755538v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1102|Milk Quality and Mastitis in Dairy Cows",
      "subfield": "plant-biology"
    }
  ],
  "title": "Macrophages from Holstein and Normande cows respond differently to Escherichia coli and Streptococcus uberis",
  "title_text": "Macrophages from Holstein and Normande cows respond differently to Escherichia coli and Streptococcus uberis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:18Z",
  "url": "https://research.draconitic.com/paper/c9kyxf5na7eng",
  "venue": "bioRxiv",
  "versions": []
}