{
  "abstract": "Elevated levels of anti-preS1 antibodies in patients correlate with faster hepatitis B virus (HBV) clearance, since preS1 is vital for viral entry. However, accurate quantification of preS1 antibodies is hindered by the variation of HBV genotypes. Herein, a quantitative preS1 antibody assay was developed for Chinese patients with predominant HBV genotypes B and C. First, to address genotype diversity, we constructed a dual recombinant protein combining genotype B and C preS1. Second, a mouse-human chimeric antibody with high and comparable affinity for both B and C preS1 is developed as the standard for quantification by screening and cloning. An optimized ELISA kit using these components demonstrated good specificity, accuracy, reproducibility and stability, with a linear range of 10-100 ng/mL. This approach extends the patient coverage in China to over 95%, providing a valuable diagnostic tool. This work demonstrates a proof-of-concept model for developing broad-coverage assays for other multi-genotype viral diseases.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "Yumin Li"
    },
    {
      "name": "Xiaoyu Wen"
    },
    {
      "name": "Jing Zhan"
    },
    {
      "name": "Tingting Cao"
    },
    {
      "name": "Xiumei Chi"
    },
    {
      "name": "Huanyi Cheng"
    },
    {
      "name": "Qingrui Huang"
    },
    {
      "name": "Wenyi Zhang"
    },
    {
      "name": "Junqi Niu"
    },
    {
      "name": "Huan Du"
    },
    {
      "name": "Zhenning Liu",
      "orcid": "0000-0001-7075-0331"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ca8afb5j93q2e.bib",
  "cited_by": 0,
  "doi": "10.1016/j.isci.2026.117857",
  "fulltext_url": "https://europepmc.org/articles/PMC13682388",
  "id": "ca8afb5j93q2e",
  "issns": [
    "2589-0042"
  ],
  "landing_url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03238-4",
  "language": "eng",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "http://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Immunology"
    ],
    [
      "heading",
      "Medical biochemistry"
    ],
    [
      "heading",
      "Virology"
    ],
    [
      "openalex",
      "2713|Hepatitis B Virus Studies"
    ]
  ],
  "openalex": "W7221622846",
  "pdf_url": "https://europepmc.org/articles/PMC13682388?pdf=render",
  "pmcid": "PMC13682388",
  "pmid": "42903824",
  "published": "2026-10-13",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/ca8afb5j93q2e.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:16Z",
      "native_id": "10.1016/j.isci.2026.117857",
      "source": "feed-cell",
      "url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03238-4"
    },
    {
      "first_seen": "2026-10-30T12:17:49Z",
      "native_id": "10.1016/j.isci.2026.117857",
      "source": "crossref",
      "url": "https://linkinghub.elsevier.com/retrieve/pii/S2589004226032384"
    },
    {
      "first_seen": "2026-10-30T12:19:53Z",
      "native_id": "MED:42903824",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42903824"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "heading:Immunology",
      "subfield": "immunology"
    },
    {
      "field": "biology",
      "origin": "heading:Virology",
      "subfield": "microbiology"
    }
  ],
  "title": "A dual recombinant strategy to quantify PreS1 antibody in hepatitis B patients with genotype B or C virus by ELISA",
  "title_text": "A dual recombinant strategy to quantify PreS1 antibody in hepatitis B patients with genotype B or C virus by ELISA",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:53Z",
  "url": "https://research.draconitic.com/paper/ca8afb5j93q2e",
  "venue": "iScience",
  "versions": []
}