{
  "abstract": "Higher-order antibody aggregates remain challenging to characterize using conventional mass spectrometry because of their low abundance, broad mass distributions, and extensive heterogeneity. Here, the practical performance of a commercial electrostatic linear ion trap charge detection mass spectrometer (XevoTM CDMS) was evaluated for therapeutic antibody analysis. The platform provided reproducible mass and charge measurements, with repeatability and intermediate-precision RSDs below 1%, and maintained consistent mass determination across the concentration and solution conditions examined. Controlled mixtures of antibody-derived species showed a linear molar-ratio-dependent ion response, supporting semi-quantitative estimation of relative species abundance under defined conditions. For heat-stressed trastuzumab, CDMS measurements were qualitatively consistent with SEC-MALS, mass photometry, and conventional native MS, while enabling direct mass determination of individual ions within highly heterogeneous high-molecular-weight (HMW) populations that exhibited extensive charge-state overlap and could not be reliably deconvoluted by conventional native MS. High-duty-cycle acquisition increased sampling of low-abundance, high-m/z ions and improved access to higher-order aggregate signals. Joint single ion mass-charge analysis further differentiated statistical ion families within the broad HMW fraction and related major mass differences to independently obtained compositional information. These results support commercial XevoTM CDMS as a practical multidimensional platform for antibody aggregate characterization.",
  "abstract_shown": true,
  "affiliations": [
    "Zhejiang University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 16,
  "authors": [
    {
      "name": "S.-Y. Wu"
    },
    {
      "name": "X.-M. Guo"
    },
    {
      "name": "J.-Y. Lv"
    },
    {
      "name": "Y.-Z. Li"
    },
    {
      "name": "L. Gu"
    },
    {
      "name": "Z. Tian"
    },
    {
      "name": "Y.-S. Xiao"
    },
    {
      "name": "E. Christofi"
    },
    {
      "name": "A. Haris"
    },
    {
      "name": "H. Lu"
    },
    {
      "name": "R. Wang"
    },
    {
      "name": "H.-F. Xie"
    },
    {
      "name": "H. Li"
    },
    {
      "name": "Y. Pan"
    },
    {
      "name": "M. Zhou"
    },
    {
      "affs": [
        0
      ],
      "name": "W.-J. Fang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cazypxc688vm7.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.08.757778",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.08.757778v1.full",
  "id": "cazypxc688vm7",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.08.757778v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1607|Mass Spectrometry Techniques and Applications"
    ]
  ],
  "openalex": "W7222313248",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.08.757778v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cazypxc688vm7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:28:00Z",
      "native_id": "10.64898/2026.10.08.757778",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.08.757778v1"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Mass Spectrometry Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Benchmarking the Performance of a Commercial Electrostatic Linear Ion Trap (ELIT) Charge Detection Mass Spectrometer For Higher-Order Antibody Aggregate Analysis",
  "title_text": "Benchmarking the Performance of a Commercial Electrostatic Linear Ion Trap (ELIT) Charge Detection Mass Spectrometer For Higher-Order Antibody Aggregate Analysis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:28:00Z",
  "url": "https://research.draconitic.com/paper/cazypxc688vm7",
  "venue": "bioRxiv",
  "versions": []
}