{
  "abstract": "The study of protein organization at synapses and their rearrangement to maintain efficient synaptic transmission is made possible by single-molecule super-resolution imaging. Here, we present a protocol for studying the organization of GABA A R subdomains at inhibitory synapses. We describe steps for cell fixation, immunolabeling, super-resolution acquisition, and data analysis using a MATLAB open-source algorithm. This protocol has potential applications in the study of membrane molecules at synapses, as well as in other cellular compartments. For complete details on the use and execution of this protocol, please refer to Merlaud et al. 1 • Instructions for implementing PALM/STORM microscopy • Steps for accessing nanoscale organization of membrane proteins via PALM/STORM imaging • Guidance on how to study GABA A R molecular organization within synapses at nanoscale Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The study of protein organization at synapses and their rearrangement to maintain efficient synaptic transmission is made possible by single-molecule super-resolution imaging. Here, we present a protocol for studying the organization of GABA A R subdomains at inhibitory synapses. We describe steps for cell fixation, immunolabeling, super-resolution acquisition, and data analysis using a MATLAB open-source algorithm. This protocol has potential applications in the study of membrane molecules at synapses, as well as in other cellular compartments.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "Tânia Lima"
    },
    {
      "name": "Anne-Lise Paupiah"
    },
    {
      "name": "Zaha Merlaud"
    },
    {
      "name": "Zahra Imani"
    },
    {
      "name": "Sabine Lévi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cb6rqgbgetxse.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2025.104031",
  "fulltext_url": null,
  "id": "cb6rqgbgetxse",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://hal.science/hal-05784047v1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.neu"
    ],
    [
      "hal",
      "2.sdv.neu.nb"
    ],
    [
      "openalex",
      "2804|Neuroscience and Neuropharmacology Research"
    ]
  ],
  "openalex": "W4413270061",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-07",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/cb6rqgbgetxse.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:50Z",
      "native_id": "hal-05784047",
      "source": "hal",
      "url": "https://hal.science/hal-05784047v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.neu",
      "subfield": "neuroscience"
    }
  ],
  "title": "Protocol for studying GABAA receptor subsynaptic domains in rat hippocampal neurons using single-molecule localization microscopy",
  "title_text": "Protocol for studying GABAA receptor subsynaptic domains in rat hippocampal neurons using single-molecule localization microscopy",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:50Z",
  "url": "https://research.draconitic.com/paper/cb6rqgbgetxse",
  "venue": "STAR Protocols",
  "versions": []
}