{
  "abstract": "N 6-methyladenosine (m6A) is a prevalent internal modification of eukaryotic mRNA that influences transcript fate, including mRNA stability and cell-type-specific gene expression. However, the mechanisms underlying m6A-mediated regulation remain poorly understood in many systems, including the highly regenerative planarian Schmidtea mediterranea. To address this, we generated a high-confidence atlas of ∼72 200 m6A sites across the planarian transcriptome using multiplexed direct RNA sequencing. The m6A sites follow a DRAYW consensus motif and are highly enriched near stop codons while being largely excluded from coding sequences. This pattern aligns with an exon and intron length-dependent variant of the exon junction complex (EJC)-mediated exclusion model, wherein the EJC restricts m6A deposition near splice sites. Knockdown of the m6A writer complex induced pronounced, cell-type-specific changes in transcript stability. Destabilized transcripts were enriched for intestinal markers, whereas stabilized transcripts were associated with neoblasts, the adult stem cells of planarians. Transcriptional shut-off experiments confirmed that m6A has opposing effects on mRNA decay depending on cellular context: it stabilizes transcripts in differentiated cells, while it promotes the degradation of mRNAs associated with neoblasts. Collectively, these results support a model in which cell-type-specific regulation of mRNA stability by m6A plays a crucial role in shaping cell identity in planarians.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "Constantin Höhn"
    },
    {
      "name": "Andreas Pittroff"
    },
    {
      "name": "Anne-Sophie Gribling-Burrer"
    },
    {
      "name": "Lisa Hülsmann"
    },
    {
      "name": "Redmond Smyth"
    },
    {
      "name": "Claus-D Kuhn"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cb7tem92wdxa4.bib",
  "cited_by": 0,
  "doi": "10.1093/nar/gkag892",
  "fulltext_url": null,
  "id": "cb7tem92wdxa4",
  "issns": [
    "0305-1048",
    "1362-4962"
  ],
  "landing_url": "https://hal.science/hal-05791574v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|RNA modifications and cancer"
    ]
  ],
  "openalex": "W7214035797",
  "pdf_url": "https://hal.science/hal-05791574/document",
  "pmcid": null,
  "pmid": "42770330",
  "published": "2026-09-22",
  "publisher": "Oxford University Press",
  "ris": "https://research.draconitic.com/paper/cb7tem92wdxa4.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:53Z",
      "native_id": "hal-05791574",
      "source": "hal",
      "url": "https://hal.science/hal-05791574v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA modifications and cancer",
      "subfield": "molecular-biology"
    }
  ],
  "title": "The stability of m6A-marked transcripts is linked to cell identity in planarians",
  "title_text": "The stability of m6A-marked transcripts is linked to cell identity in planarians",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T12:20:48Z",
  "url": "https://research.draconitic.com/paper/cb7tem92wdxa4",
  "venue": "Nucleic Acids Research",
  "versions": []
}