{
  "abstract": "Etioplasts of dark-grown angiosperm seedlings contain prolamellar bodies (PLBs), lattice-like membranes that are quickly transformed into thylakoids upon illumination. Phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG) are major anionic lipids in these membranes, but their roles in etioplast-to-chloroplast differentiation remain unclear. We analyzed Arabidopsis thaliana mutants with reduced PG biosynthesis (pgp1-1), complete SQDG deficiency (sqd1), and the pgp1-1 sqd1 double mutant. Although pgp1-1 etioplasts showed a loosened PLB lattice when fixed under white light, they retained regular, wild-type-like lattices when fixed under infrared light, suggesting that their PLBs were more susceptible to light-induced disassembly. The rapid PLB disassembly in pgp1-1 upon illumination was accompanied by rapid conversion of dark-accumulated protochlorophyllide to chlorophyll and lamellar membrane formation, suggesting a link between membrane remodeling and chlorophyll formation. However, subsequent chlorophyll accumulation through de novo biosynthesis was retarded in illuminated pgp1-1 seedlings, whereas photosynthetic electron transport was quickly activated. Although sqd1 showed no obvious defects, pgp1-1 sqd1 exhibited more severe defects than pgp1-1, particularly in internal membrane formation, chlorophyll accumulation, photosynthetic activation, and photosynthetic gene expression. These findings suggest that PG is crucial for membrane-associated processes during etioplast-to-chloroplast differentiation, while SQDG may help maintain chloroplast development when PG availability is limited.",
  "abstract_shown": true,
  "affiliations": [
    "Osaka Metropolitan University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "A. Yoshihara"
    },
    {
      "name": "R. Uwatoko"
    },
    {
      "affs": [
        0
      ],
      "name": "K. Kobayashi"
    },
    {
      "name": "N. Nagata"
    },
    {
      "affs": [
        0
      ],
      "name": "K. Kobayashi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cb8ktq6bctbrv.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.31.763292",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.31.763292v1.full",
  "id": "cb8ktq6bctbrv",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.31.763292v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.31.763292v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-31",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cb8ktq6bctbrv.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:21:05Z",
      "native_id": "10.64898/2026.10.31.763292",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.31.763292v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "Anionic lipids stabilize etioplast prolamellar bodies and facilitate thylakoid biogenesis during etioplast-to-chloroplast differentiation",
  "title_text": "Anionic lipids stabilize etioplast prolamellar bodies and facilitate thylakoid biogenesis during etioplast-to-chloroplast differentiation",
  "type": "preprint",
  "updated_at": "2026-11-06T00:20:51Z",
  "url": "https://research.draconitic.com/paper/cb8ktq6bctbrv",
  "venue": "bioRxiv",
  "versions": []
}