{
  "abstract": "Background: Rheumatoid arthritis (RA)-associated bone erosion is largely driven by excessive osteoclast differentiation and activation. Total saponins of Panax japonicus C.A. Meyer (TSPJs), a ginseng-derived botanical fraction, show anti-inflammatory activity in RA-related settings, but their effects on osteoclast-mediated bone destruction remain unclear. Methods: Transcriptomic datasets from RA synovial tissues and RANKL-induced osteoclast differentiation were integrated to identify shared pathogenic signals. TSPJs were chemically profiled by HPLC and evaluated in collagen-induced arthritis mice and RANKL-stimulated RAW264.7 cells. Osteoclast formation and function were assessed by TRAP staining, F-actin ring analysis, gene expression, bone resorption assays, histopathology, radiography, and serological measurements. Network pharmacology, molecular docking, CETSA, immunofluorescence, Western blotting, and immunohistochemistry were used to explore candidate mechanisms. Results: Integrated bioinformatics highlighted NF-κB-related signaling as a shared pathway linking RA and osteoclastogenesis. HPLC identified four representative saponins: araloside A, chikusetsusaponin IVa, ginsenoside Rg2, and ginsenoside Ro. In CIA mice, TSPJs reduced arthritis severity, improved joint pathology, lowered osteoclast-associated serum markers, and attenuated radiographic and histological bone erosion. In vitro, TSPJs inhibited RANKL-induced osteoclast differentiation, disrupted F-actin rings, downregulated osteoclast-related genes, and reduced bone resorption. The overall evidence was consistent with modulation of SRC/TRAF6/NF-κB signaling, while docking and CETSA supported SRC/NFATc1/FOS-related nodes as candidates for further validation. Conclusion: TSPJs attenuated RA-associated bone erosion and restrained osteoclastogenesis, supporting further evaluation of this Panax japonicus -derived saponin preparation.",
  "abstract_shown": true,
  "affiliations": [
    "Basic Medical College of China Three Gorges University, Yichang, Hubei, 443002, China; Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by the State Administration of Traditional Chinese Medicine, College of Medicine and Health Science of China Three Gorges University, Yichang, Hubei, 443002, China",
    "Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by the State Administration of Traditional Chinese Medicine, College of Medicine and Health Science of China Three Gorges University, Yichang, Hubei, 443002, China",
    "The Second Clinical Medical School, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China",
    "The Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China",
    "Institute of Rheumatology, the First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei, 443003, China; Corresponding author. The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei, 443003, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
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        0
      ],
      "name": "Mei Yang"
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    {
      "affs": [
        1
      ],
      "name": "Jingkai Zhang"
    },
    {
      "affs": [
        2
      ],
      "name": "Xiang Guo"
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    {
      "affs": [
        1
      ],
      "name": "Yushen Chen"
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    {
      "affs": [
        3
      ],
      "name": "Zhigang Mei"
    },
    {
      "affs": [
        4
      ],
      "name": "Xiaoqiang Hou"
    },
    {
      "name": "Zhitao Feng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ccjwqgh9bhp9e.bib",
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  "doi": "10.1016/j.jgr.2026.101113",
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    "2093-4947"
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  "landing_url": "https://doi.org/10.1016/j.jgr.2026.101113",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
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  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
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  "pdf_url": "https://europepmc.org/articles/PMC13711408?pdf=render",
  "pmcid": "PMC13711408",
  "pmid": "42948600",
  "published": "2026-07-11",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/ccjwqgh9bhp9e.ris",
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Bone Metabolism and Diseases",
      "subfield": "molecular-biology"
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  "title": "Chemically profiled total saponins of",
  "title_text": "Chemically profiled total saponins of",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:22:11Z",
  "url": "https://research.draconitic.com/paper/ccjwqgh9bhp9e",
  "venue": "Journal of Ginseng Research",
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}