{
  "abstract": "Introduction: Progressive decline in functional pancreatic β-cell mass is a key pathological feature of type 2 diabetes mellitus (T2DM), highlighting the need for therapies that preserve and regenerate β-cells. This study investigated the preservative effects of a chicoric acid-rich aqueous extract of Cichorium intybus seeds (CAREOCI) under glucotoxic conditions (25 mM glucose) in pancreatic β-cells.\n\nMethods: Phytochemical standardisation by HPTLC, HPLC, and LC–MS/MS confirmed chicoric acid is the principal constituent present in CAREOCI. A molecular docking study identified DYRK1A is a one of the key target (−8.72 kcal/mol) of chicoric acid for β-cell regeneration. In glucotoxic MIN 6 β-cells, CAREOCI significantly enhanced glucose-stimulated insulin secretion, restored antioxidant status, reduced intracellular ROS, and suppressed IL-1β and TNF-α.\n\nResults: CAREOCI also reduced apoptosis, attenuated caspase-3/7 activity, preserved normal nuclear morphology, restored mitochondrial membrane potential, and promoted autophagy through increased acidic vesicular organelles. Cell-cycle analysis indicated a trend toward increased S-phase population, suggesting enhanced β-cell proliferation. At the molecular level, CAREOCI downregulated DYRK1A, NF-κB, mTOR, and CHOP while upregulating MAFA, PDX1, PAX6, and NKX6.1, supporting β-cell survival and preservation. Compared with purified chicoric acid, CAREOCI consistently exhibited greater efficacy, indicating potential complementarity among its phytoconstituents.\n\nConclusion: CAREOCI protects pancreatic β-cells through coordinated antioxidant, anti-inflammatory, anti-apoptotic, mitochondrial-protective, autophagy-promoting effects, supporting beta cell preservation and function under glucotoxicity condition supporting its potential as a phytopharmaceutical for T2DM.",
  "abstract_shown": true,
  "affiliations": [
    "Hamdard Institute of Medical Sciences and Research (HIMSR) , ,",
    "Jamia Hamdard School of Chemical and Life Sciences , ,",
    "Jamia Hamdard School of Pharmaceutical Education and Research , ,",
    "ICMR - National Institute of Cancer Prevention and Research , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Anish Murtaja Alam Khan"
    },
    {
      "affs": [
        0
      ],
      "name": "Kailash Chandra"
    },
    {
      "affs": [
        1
      ],
      "name": "Furqan Choudhary"
    },
    {
      "affs": [
        2
      ],
      "name": "Aftab Alam"
    },
    {
      "affs": [
        2
      ],
      "name": "Farheen Azad"
    },
    {
      "affs": [
        2
      ],
      "name": "Jasmeet Kaur"
    },
    {
      "affs": [
        0
      ],
      "name": "Mohit Suyal"
    },
    {
      "affs": [
        3
      ],
      "name": "Yasmin Bano"
    },
    {
      "affs": [
        3
      ],
      "name": "Dinesh Kumar Singhal"
    },
    {
      "affs": [
        2
      ],
      "name": "Sayeed Ahmad"
    },
    {
      "affs": [
        2
      ],
      "name": "Bibhu Prasad Panda"
    },
    {
      "affs": [
        0
      ],
      "name": "Vineet Jain"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ccnewss0555sw.bib",
  "cited_by": 0,
  "doi": "10.3389/fphar.2026.1953632",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13661457/?tool=EBI",
  "id": "ccnewss0555sw",
  "issns": [
    "1663-9812"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13661457",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2746|Pancreatic function and diabetes"
    ]
  ],
  "openalex": "W7214953715",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13661457/pdf/?tool=EBI",
  "pmcid": "PMC13661457",
  "pmid": null,
  "published": "2026-09-30",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/ccnewss0555sw.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:21Z",
      "native_id": "PMC:PMC13661457",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13661457"
    },
    {
      "first_seen": "2026-10-30T12:20:06Z",
      "native_id": "10.3389/fphar.2026.1953632",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fphar.2026.1953632/full"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2746|Pancreatic function and diabetes",
      "subfield": "surgery"
    }
  ],
  "title": "Chicoric acid-rich extract of Cichorium intybus seeds (CAREOCI) preserves pancreatic β-cell function under glucotoxic stress through modulation of MAFA, DYRK1A, cell cycle, apoptosis, and mitochondrial dysfunction",
  "title_text": "Chicoric acid-rich extract of Cichorium intybus seeds (CAREOCI) preserves pancreatic β-cell function under glucotoxic stress through modulation of MAFA, DYRK1A, cell cycle, apoptosis, and mitochondrial dysfunction",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:06Z",
  "url": "https://research.draconitic.com/paper/ccnewss0555sw",
  "venue": "Frontiers in pharmacology",
  "versions": []
}