{
  "abstract": "Trimerization motifs play pivotal roles in structural biology and therapeutic protein engineering. Here, we describe a short trimerization motif (rFd1303) derived from the Reoviridae family reovirus σ1 protein. Compared with the widely used T4-Foldon, the rFd1303 increased recombinant protein yields. The rFd1303-fused immunogens of SARS-CoV-2 spike and influenza hemagglutinin elicited antibody responses comparable to T4-Foldon-fused controls in mice. Using this tag, we engineered a trimeric ACE2-Ig fusion protein (TriACE2-Ig) that remained stable after 30 days at room temperature and neutralized a broad range of ACE2-utilizing sarbecoviruses, including 19 SARS-CoV-2 variants, SARS-CoV, and pangolin coronaviruses GD and GX, with higher potency than monomeric ACE2-Ig. In the hamster model challenged with various SARS-CoV-2 variants, our data demonstrated that intranasal TriACE2-Ig administration markedly reduced viral loads, virus-induced body-weight loss, lung pathology, and decreased within-cage virus transmission. These findings highlight rFd1303 as a versatile trimerization platform for vaccine and therapeutic protein development.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, PR China",
    "National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Center of Biologic Products, National Innovation Platform for Industry-Education Integration in Vaccine Research, Xiamen University, Xiamen, PR China",
    "State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, PR China",
    "Joint Institute of Virology (Shantou University and The University of Hong Kong), Guangdong-Hongkong Joint Laboratory of Emerging Infectious Diseases, Shantou University, Shantou, PR China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 37,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Min Wei"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Qingfang Bu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jin Xiao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Lunzhi Yuan"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jinmiao Shi"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Huilin Guo"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yang Huang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hui Sun",
      "orcid": "0009-0006-9866-9846"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Kai Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zonglin Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Min Lin"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhaoming Lu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Chen Zheng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jijing Chen"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jiahua Gao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zikang Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zehong Huang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ruojing Bai"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ying Liu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jian Ma"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yunda Hong"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Youfeng Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jiayi Wu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yifan Yin"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Shengxiang Ge",
      "orcid": "0000-0002-7165-3004"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zizheng Zheng",
      "orcid": "0000-0003-0099-4212"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yixin Chen",
      "orcid": "0000-0002-9591-634X"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Yi Guan",
      "orcid": "0000-0001-6057-9243"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hai Yu",
      "orcid": "0000-0003-4086-8632"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Tianying Zhang",
      "orcid": "0000-0002-2477-329X"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jun Zhang",
      "orcid": "0000-0002-6601-9180"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Shaojuan Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Qingbing Zheng",
      "orcid": "0000-0002-7516-9965"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yangtao Wu",
      "orcid": "0000-0002-9429-266X"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ningshao Xia",
      "orcid": "0000-0003-0179-5266"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yali Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Quan Yuan",
      "orcid": "0000-0001-5487-561X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cd0wgvtzr33kp.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-77926-x",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13696707/",
  "id": "cd0wgvtzr33kp",
  "issns": [
    "2041-1723"
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  "landing_url": "https://doi.org/10.1038/s41467-026-77926-x",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
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    [
      "heading",
      "Article"
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      "2725|SARS-CoV-2 and COVID-19 Research"
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  "pdf_url": "https://europepmc.org/articles/PMC13696707?pdf=render",
  "pmcid": "PMC13696707",
  "pmid": "42928093",
  "published": "2026-09-24",
  "publisher": "Nature Publishing Group",
  "ris": "https://research.draconitic.com/paper/cd0wgvtzr33kp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:01Z",
      "native_id": "PMC13696707",
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      "url": "https://doi.org/10.1038/s41467-026-77926-x"
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|SARS-CoV-2 and COVID-19 Research",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Reoviridae-derived short trimerization domain for stabilizing homo-trimerization of vaccine immunogens and therapeutic proteins",
  "title_text": "Reoviridae-derived short trimerization domain for stabilizing homo-trimerization of vaccine immunogens and therapeutic proteins",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:34:00Z",
  "url": "https://research.draconitic.com/paper/cd0wgvtzr33kp",
  "venue": "Nature Communications",
  "versions": []
}