{
  "abstract": "Pulse Dipolar Electron Paramagnetic Resonance Spectroscopy (PDS) is a powerful method for determining nanometer distances in biomolecular systems. This methodology has been successfully used to validate structure hypotheses and to identify disorder. However, inherent distance limitations are under-explored. Many challenging biomolecular applications require long distance information, driving the need for a benchmarking study. We demonstrate distances up to 10.5 nm in TRIM25 reliably extracted from data obtained under standard sample conditions. By protein perdeuteration accurate distance measurements up to 17.3 nm were enabled. Enhancing sensitivity through optimization of the measurement and sample preparation conditions the achievable dipolar evolution time was extended to 150 μs. Thus, distances close to 20 nm should become experimentally accessible, highlighting the potential of advanced PDS methodologies for probing previously inaccessible long-range structural information in complex systems.",
  "abstract_shown": true,
  "affiliations": [
    "EaStCHEM School of Chemistry, Biomedical Sciences Research Complex and Centre of Magnetic Resonance, University of St.Andrews, North Haugh, St AndrewsKY16 9ST, 248 ScotlandUK.",
    "Clausius-Institute for Physical and Theoretical Chemistry, University of Bonn, Wegelerstr. 12, 53115Bonn, Germany.",
    "School of Life Sciences, University of Dundee, Medical Sciences Institute, Nethergate DundeeDD1 4HN, Scotland, UK."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Yannik Limbach"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Katrin Ackermann",
      "orcid": "0000-0003-1632-0503"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Aisika Chakraborty"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "David G Norman",
      "orcid": "0000-0002-7658-7720"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Bela E Bode",
      "orcid": "0000-0002-3384-271X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ce58jhkbxqsm7.bib",
  "cited_by": 0,
  "doi": "10.1021/acs.jpclett.6c02671",
  "fulltext_url": "https://europepmc.org/articles/PMC13679085",
  "id": "ce58jhkbxqsm7",
  "issns": [
    "1948-7185"
  ],
  "landing_url": "https://europepmc.org/article/MED/42918236",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1304|Electron Spin Resonance Studies"
    ]
  ],
  "openalex": "W7220402748",
  "pdf_url": "https://europepmc.org/articles/PMC13679085?pdf=render",
  "pmcid": "PMC13679085",
  "pmid": "42918236",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ce58jhkbxqsm7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42918236",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42918236"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Electron Spin Resonance Studies",
      "subfield": "biophysics"
    }
  ],
  "title": "Spins Afar─Long-Range Pulse Dipolar EPR Spectroscopy Uncovers Curvature in TRIM25's Coiled Coil.",
  "title_text": "Spins Afar─Long-Range Pulse Dipolar EPR Spectroscopy Uncovers Curvature in TRIM25's Coiled Coil.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/ce58jhkbxqsm7",
  "venue": "The journal of physical chemistry letters",
  "versions": []
}