{
  "abstract": "Cell-surface glycoRNAs participate in diverse biological processes, but they were investigated as pooled populations and the protein interactome of individual glycoRNA species remain unresolved. Here we develop GlycoRNA-Map, a programmable photocatalytic proximity-labeling method that maps proteins surrounding sequence-defined endogenous glycoRNAs on living cells. A sialic-acid-binding aptamer and an RNA-hybridization probe jointly recruit a fluorophore-modified oligonucleotide through dual recognition of the glycan and RNA moieties of glycoRNAs. Light-activated biotinylation followed by quantitative mass spectrometry identified 200-418 enriched proteins for each of five glycoRNAs in A549 cells. Comparison of these proteomes revealed limited overlap among different glycoRNA sequences and across cell types, demonstrating that glycoRNA-associated protein environments depend on both RNA identity and cellular context. Transforming growth factor-{beta} receptors 1 and 2 (TGFBR1 and TGFBR2) were identified in the proximal proteomes of multiple glycoRNAs. Removal of cell-surface RNAs enhanced TGF-{beta} receptor signaling and promoted cell migration, demonstrating that glycoRNAs can functionally regulate extracellular signaling by interacting with their protein neighborhoods. GlycoRNA-Map thus provides an accessible strategy for resolving sequence-specific glycoRNA-protein interactome and discovering their roles in membrane signaling.",
  "abstract_shown": true,
  "affiliations": [
    "Rice University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "name": "X. Zhang"
    },
    {
      "name": "Y. Zhu"
    },
    {
      "name": "K. Baumer"
    },
    {
      "name": "Y. Lu"
    },
    {
      "name": "Y. Fu"
    },
    {
      "name": "J. Liao"
    },
    {
      "name": "X. Shao"
    },
    {
      "name": "Y. Chang"
    },
    {
      "name": "E. Qiao"
    },
    {
      "name": "H. Dong"
    },
    {
      "name": "A. S. Valverde"
    },
    {
      "name": "H.-C. Yeh"
    },
    {
      "name": "S.-H. Chen"
    },
    {
      "name": "H. Renata"
    },
    {
      "affs": [
        0
      ],
      "name": "Y. Ma"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ceabq34ejegfa.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.29.755375",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755375v1.full",
  "id": "ceabq34ejegfa",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755375v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1307|Biotin and Related Studies"
    ]
  ],
  "openalex": "W7214935539",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755375v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ceabq34ejegfa.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:25:24Z",
      "native_id": "10.64898/2026.09.29.755375",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.29.755375v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Biotin and Related Studies",
      "subfield": "cell-biology"
    }
  ],
  "title": "Programmable sequence-resolved in situ profiling of glycoRNA-protein interactomes by GlycoRNA-Map",
  "title_text": "Programmable sequence-resolved in situ profiling of glycoRNA-protein interactomes by GlycoRNA-Map",
  "type": "preprint",
  "updated_at": "2026-10-30T12:25:28Z",
  "url": "https://research.draconitic.com/paper/ceabq34ejegfa",
  "venue": "bioRxiv",
  "versions": []
}