{
  "abstract": "Granulosa cells (GCs) critically govern follicular development through the coordinated regulation of proliferation, apoptosis, and steroidogenesis. Neuromedin B (NMB), a local regulatory peptide, has been implicated in female reproductive processes. However, its precise effects on porcine GCs remain to be elucidated. In this study, we characterized the ovarian distribution of NMBR, investigated the effects of NMB on GC function, and further explored the involvement of NMBR in these effects using the NMBR antagonist PD168368. The results showed that higher NMBR expression was observed in follicles of larger diameter. NMB treatment differentially regulated the functional activities of porcine ovarian GCs. Specifically, certain concentrations of NMB significantly increased cell viability and altered the expression levels of the proliferation-related markers Cyclin B1 and PCNA; reduced the cell apoptosis rate and modulated the expression levels of apoptosis-related genes and proteins (Bax, Bcl-2, and Caspase-3); and affected steroidogenic responses, including increased E₂ concentrations at selected concentrations while altering expression of STAR, CYP11A1, HSD3B1, and CYP19A1. Importantly, several of these effects were attenuated by PD168368 treatment. Collectively, these findings suggest that NMB may regulate multiple functions of porcine with the involvement of NMBR. These findings provide a theoretical basis for elucidating the neuroendocrine mechanisms underlying porcine follicular development and for further investigating the potential role of NMB/NMBR signaling in porcine follicular physiology.",
  "abstract_shown": true,
  "affiliations": [
    "College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.",
    "Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.",
    "Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Zhiyu Ma",
      "orcid": "0009-0001-5934-4700"
    },
    {
      "affs": [
        0
      ],
      "name": "Hongxia Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Guangzhen Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Jia Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Dandan Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yiwen Wang"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Sheng Yang"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Qi Liu"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Fenglei Chen",
      "orcid": "0000-0002-7433-5474"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Shifeng Pan"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cerwn0yvcm56r.bib",
  "cited_by": 0,
  "doi": "10.3390/vetsci13101088",
  "fulltext_url": "https://europepmc.org/articles/PMC13709793",
  "id": "cerwn0yvcm56r",
  "issns": [
    "2306-7381"
  ],
  "landing_url": "https://europepmc.org/article/MED/42944182",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1102|Reproductive Physiology in Livestock"
    ]
  ],
  "openalex": "W7221796070",
  "pdf_url": "https://europepmc.org/articles/PMC13709793?pdf=render",
  "pmcid": "PMC13709793",
  "pmid": "42944182",
  "published": "2026-10-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cerwn0yvcm56r.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42944182",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42944182"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1102|Reproductive Physiology in Livestock",
      "subfield": "plant-biology"
    }
  ],
  "title": "Neuromedin B Regulates Cell Proliferation, Apoptosis and Steroidogenesis of Porcine Ovarian Granulosa Cells via NMBR.",
  "title_text": "Neuromedin B Regulates Cell Proliferation, Apoptosis and Steroidogenesis of Porcine Ovarian Granulosa Cells via NMBR.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/cerwn0yvcm56r",
  "venue": "Veterinary sciences",
  "versions": []
}