{
  "abstract": "Brefeldin A (BFA)-resistant unconventional protein secretion (UPS) is documented in cellular systems across taxa and has roles in disease, but mechanistic details remain sparse. The blast fungus Magnaporthe oryzae uses Golgi-bypass UPS to deploy plant immunity-suppressing cytoplasmic effector proteins into host rice cells to establish infection. However, few M. oryzae UPS components are known, and connections to other cellular processes are undefined. Here, using live-cell imaging of M. oryzae mutant strains, we show that MoGrh1, a resident of the pre-Golgi, BFA-resistant ER-Golgi intermediate compartment (ERGIC), is required for UPS cargo sorting under the autophagy-inducing conditions of early host cell colonization. The loss of MoGRH1 abolished UPS, with cytoplasmic effectors misrouted through a BFA-sensitive secretion pathway instead. Treatment with autophagy-suppressing -ketoglutarate, a preferred carbon source, reconstituted unconventional secretion in {Delta}grh1, restoring fungal growth in host cells. We conclude that MoGrh1 supports UPS cargo sorting under the nutrient-limiting conditions of early host cell infection, linking the ERGIC and autophagy to disease-causing effector secretion in a devastating plant pathogen.",
  "abstract_shown": true,
  "affiliations": [
    "University of Nebraska-Lincoln"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "name": "N. Dulal"
    },
    {
      "affs": [
        0
      ],
      "name": "R. A. Wilson"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cfrytsq5aam0t.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.06.757164",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.06.757164v1.full",
  "id": "cfrytsq5aam0t",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.06.757164v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ],
    [
      "openalex",
      "1307|Cellular transport and secretion"
    ]
  ],
  "openalex": "W7220861222",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.06.757164v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-06",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cfrytsq5aam0t.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:28Z",
      "native_id": "10.64898/2026.10.06.757164",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.06.757164v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "Blast fungus unconventional effector secretion sorting requires pre-Golgi compartment protein MoGrh1",
  "title_text": "Blast fungus unconventional effector secretion sorting requires pre-Golgi compartment protein MoGrh1",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:28Z",
  "url": "https://research.draconitic.com/paper/cfrytsq5aam0t",
  "venue": "bioRxiv",
  "versions": []
}