{
  "abstract": "In microbial electrochemical technologies, Shewanella oneidensis MR-1 efficiently generates electric current via biofilm formation on electrodes. A previous study demonstrated that the deletion of the diguanylate cyclase gene dgcS inhibits biofilm formation on electrodes and current generation in MR-1. However, the molecular mechanisms by which dgcS expression and subsequent cyclic dimeric guanosine monophosphate (c-di-GMP) signaling affect these processes remain unexplored. Here, we investigated the effects of dgcS overexpression on current generation, electrode-associated biofilm formation, and the intracellular concentration of cyclic AMP (cAMP), a second messenger that induces the expression of extracellular electron transfer (EET)-related genes in MR-1, to examine the potential coordinated regulation of intracellular c-di-GMP and cAMP levels. In an electrochemical flow cell (EFC), the dgcS -overexpressing ( dgcS -OE) strain exhibited significantly higher intracellular c-di-GMP concentrations than the vector control (VC) strain, forming more biomass-rich biofilms on the electrode and generating higher current. However, current generation normalized to electrode-associated biomass did not differ significantly between the dgcS -OE and VC strains, suggesting that dgcS overexpression did not increase EET activity per biomass. Moreover, intracellular cAMP levels were not significantly different between the dgcS -OE and VC strains, and the transcription levels of adenylate cyclase (cAMP synthase) genes and EET-related genes in the dgcS -OE strain tended to be reduced to 0.3- to 0.6-fold of those in the VC strain. Collectively, these findings suggest that dgcS overexpression and the associated elevation of intracellular c-di-GMP levels enhance the electrogenic performance of MR-1 primarily by promoting biofilm development on electrodes, while weakly repressing the transcription of genes related to cAMP signaling.",
  "abstract_shown": true,
  "affiliations": [
    "School of Life Sciences, Tokyo University of Pharmacy and Life Sciences",
    "Faculty of Life Sciences, Toyo University"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Nanae Iwanami"
    },
    {
      "affs": [
        0
      ],
      "name": "Akiho Matsumoto"
    },
    {
      "affs": [
        1
      ],
      "name": "Ryotaro Yamamoto"
    },
    {
      "affs": [
        0
      ],
      "name": "Keisuke Tomita"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Atsushi Kouzuma"
    },
    {
      "affs": [
        0
      ],
      "name": "Kazuya Watanabe"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cg22a7zdarmg7.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1957821",
  "fulltext_url": "https://europepmc.org/articles/PMC13713693",
  "id": "cg22a7zdarmg7",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1957821/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2305|Microbial Fuel Cells and Bioremediation"
    ]
  ],
  "openalex": "W7221813512",
  "pdf_url": "https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2026.1957821/pdf",
  "pmcid": "PMC13713693",
  "pmid": "42953010",
  "published": "2026-10-14",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/cg22a7zdarmg7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.3389/fmicb.2026.1957821",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1957821/full"
    },
    {
      "first_seen": "2026-10-31T00:20:41Z",
      "native_id": "MED:42953010",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42953010"
    },
    {
      "first_seen": "2026-10-31T12:24:12Z",
      "native_id": "PMC13713693",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fmicb.2026.1957821"
    }
  ],
  "subjects": [
    {
      "field": "earth",
      "origin": "openalex:2305|Microbial Fuel Cells and Bioremediation",
      "subfield": "environmental-science"
    }
  ],
  "title": "Overexpression of the endogenous diguanylate cyclase gene dgcS enhances biofilm formation by Shewanella oneidensis MR-1 on electrodes",
  "title_text": "Overexpression of the endogenous diguanylate cyclase gene dgcS enhances biofilm formation by Shewanella oneidensis MR-1 on electrodes",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:21:19Z",
  "url": "https://research.draconitic.com/paper/cg22a7zdarmg7",
  "venue": "Frontiers in Microbiology",
  "versions": []
}