{
  "abstract": "Non-canonical Wnt pathways provide directional cues for cell polarity, but the mechanisms that translate these cues into asymmetric cellular organisation remain incompletely defined. We previously showed that a non-canonical EGL-20/Wnt-CAM-1/Ror-DSH-1/Dishevelled pathway establishes polarised membrane compartments in Caenorhabditis elegans body wall muscles. However, how EGL-20/Wnt signalling is translated into muscle polarity remains unknown. Here, we identify PZLS-1, the C. elegans ortholog of human PDZRN3, PDZRN4 and PDZD4, as a regulator of muscle polarity. Although PDZRN proteins are known targets of non-canonical Wnt-Ror signalling, how they are spatially organised within polarised cells and how their protein domains contribute to their function remain largely unknown. We reveal that PZLS-1 is itself asymmetrically localised by Wnt-Ror-Dvl signalling, and is closely associated with DSH-1. PZLS-1 and DSH-1 colocalise in intracellular puncta and are mutually required for their localisation. In vivo structure-function analyses reveal conserved protein motifs that differentially control PZLS-1 localisation, abundance and function. This includes a previously uncharacterized highly conserved sequence that regulates PZLS-1 abundance in a DSH-1-dependent manner. We further show that PZLS 1 and DSH-1 control the abundance and polarised localisation of VAB-8/KIF26b, another conserved target of Wnt-Ror signalling. Together, these findings indicate that Wnt-Ror-Dvl signalling can control the spatial organisation and abundance of PDZRN- and KIF26B-family proteins in vivo, providing a mechanism to translate extracellular polarity cues into intracellular asymmetry.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "Elise Cheynet"
    },
    {
      "name": "Sandra Duperrier"
    },
    {
      "name": "Thomas Boulin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cg2qjwp6tyszg.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.09.757662",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.09.757662v1.full",
  "id": "cg2qjwp6tyszg",
  "issns": [],
  "landing_url": "https://hal.science/hal-05826253v1",
  "language": "en",
  "licence": "CC-BY-ND",
  "licence_label": "CC BY-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bc"
    ],
    [
      "openalex",
      "1312|Wnt/β-catenin signaling in development and cancer"
    ]
  ],
  "openalex": "W7221588294",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.09.757662v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-09",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cg2qjwp6tyszg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:00Z",
      "native_id": "hal-05826253",
      "source": "hal",
      "url": "https://hal.science/hal-05826253v1"
    },
    {
      "first_seen": "2026-10-30T12:27:34Z",
      "native_id": "10.64898/2026.10.09.757662",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.09.757662v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "PZLS-1/PDZRN controls muscle polarity downstream of Wnt-Ror-Dvl signalling in C. elegans muscles",
  "title_text": "PZLS-1/PDZRN controls muscle polarity downstream of Wnt-Ror-Dvl signalling in C. elegans muscles",
  "type": "preprint",
  "updated_at": "2026-10-30T12:27:34Z",
  "url": "https://research.draconitic.com/paper/cg2qjwp6tyszg",
  "venue": "HAL",
  "versions": []
}