{
  "abstract": "Cold-adapted lipases have attracted extensive research attention in enzymology owing to their unique low-temperature adaptability and catalytic properties, and investigations in this area continue to deepen. This study aimed to efficiently produce a cold-adapted lipase to overcome the limitations of conventional lipases under low-temperature conditions, such as low activity, high cost, and restricted applicability. Fungal strains were isolated from naturally cold environments, and subjected to multiple rounds of screening for lipase activity. A selected strain was further improved by UV-nitrosoguanidine (NTG) combined mutagenesis, followed by optimization of fermentation conditions. The lipasewas purified by ammonium sulfate precipitation, Q-HP anion exchange chromatography, and Superdex 75 gel filtration chromatography, and its enzymatic properties were characterized. A lipase-producing strain identified as Aspergillus niger was obtained through screening. Following combined mutagenesis, lipase activity by the mutant strain increased by 127.35%. Under the optimized fermentation conditions (temperature 21℃, pH 6.0, inoculation size 6.5 × 10 ⁶ spores/mL), the lipase activity further increased to 57.26 U/mL. After purification, the cold-adapted lipase exhibited a specific activity of 113.52 U/mg. Its optimal reaction temperature was 30℃ and pH 7.0 and retained more than 60% of its maximum activity at 15℃. Its high relative activity at low temperatures, together with its low thermal stability, supported its classification as a cold-adapted lipase. This purification strategy can produce high-purity lipase at laboratory scale, which provides a pure enzyme preparation for biochemical studies. The cold-adapted lipase obtained in this study may have promising application prospects in fields such as food processing, low-temperature laundry, and environmental management.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Lutong Yang"
    },
    {
      "name": "Mengai Wang"
    },
    {
      "name": "Hongyan Fu"
    },
    {
      "name": "Yun Zhang"
    },
    {
      "name": "Yu Zhang"
    },
    {
      "name": "Mengni Cui"
    },
    {
      "name": "Bowen Jiang"
    },
    {
      "name": "Hong Chen"
    },
    {
      "name": "Yanwei Cui"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cgq5x48rn7ysp.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-74081-7",
  "fulltext_url": null,
  "id": "cgq5x48rn7ysp",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-74081-7",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1305|Enzyme Production and Characterization"
    ]
  ],
  "openalex": "W7166584123",
  "pdf_url": "https://www.nature.com/articles/s41598-026-74081-7_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-05",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/cgq5x48rn7ysp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:22Z",
      "native_id": "10.1038/s41598-026-74081-7",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-74081-7"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1305|Enzyme Production and Characterization",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Breeding of high-yield cold-adapted lipase fungi, optimization of fermentation conditions and isolation and purification of lipase",
  "title_text": "Breeding of high-yield cold-adapted lipase fungi, optimization of fermentation conditions and isolation and purification of lipase",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:22Z",
  "url": "https://research.draconitic.com/paper/cgq5x48rn7ysp",
  "venue": "Scientific Reports",
  "versions": [
    {
      "doi": "10.21203/rs.3.rs-9989697/v1",
      "pid": null,
      "relation": "preprint",
      "title": null,
      "venue": null
    }
  ]
}