{
  "abstract": "The Wuzhishan (WZS) minipig, native to Hainan Province, China, is valuable for both agriculture and biomedicine, creating a need for a telomere-to-telomere (T2T) genome assembly to support breeding and biomedical research. In this study, a gap-free, T2T genome assembly organized into 20 chromosomes was generated for the WZS pig (designated WZS-T2T) using a combination of PacBio HiFi, Oxford Nanopore ultra-long reads, and Hi-C data. The assembly spans approximately 2.68 Gb with a scaffold N50 of approximately 142.87 Mb. High completeness and accuracy were demonstrated by a Quality Value (QV) score of 70.2 and a BUSCO completeness score of 99.3%. A total of 23,100 protein-coding genes were predicted, and telomeres were fully assembled across all 20 chromosomes. Notably, the Y chromosome (ChrY) represents the longest assembled ChrY among published T2T pig genomes. Comparative analyses showed that sex differentiation-related genes (including SRY) and innate immune-related genes (PRKX and DDX3X) are conserved across most porcine Y chromosomes. Conversely, the energy metabolism gene SLC25A4 and the macrophage receptor-related gene CSF2RA were identified exclusively in the WZS-T2T assembly among the analyzed genomes. These gene-content variations on ChrY likely reflect both individual variation and differences in assembly completeness. Overall, a high-quality WZS reference genome was established to support agricultural and biomedical applications.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences , Haikou, Hainan 571100 ,",
    "Sanya Institute, Hainan Academy of Agricultural Sciences , Sanya, Hainan 572025 ,",
    "College of Animal Sciences, Fujian Agriculture and Forestry University , Fuzhou, Fujian 350002 ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yuwei Ren"
    },
    {
      "affs": [
        0
      ],
      "name": "Feng Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xinjian Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Guangliang Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Ruiping Sun"
    },
    {
      "affs": [
        0
      ],
      "name": "Xinli Zheng"
    },
    {
      "affs": [
        0
      ],
      "name": "Yan Zhang"
    },
    {
      "affs": [
        2
      ],
      "name": "Ruiyi Lin"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Xuyang Lu"
    },
    {
      "affs": [
        0
      ],
      "name": "Linlin Chen"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Wenshui Xin"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhe Chao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ch5wrc84pkn71.bib",
  "cited_by": 0,
  "doi": "10.1093/g3journal/jkag295",
  "fulltext_url": null,
  "id": "ch5wrc84pkn71",
  "issns": [
    "2160-1836"
  ],
  "landing_url": "https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag295/8979769",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Genomics and Phylogenetic Studies"
    ]
  ],
  "openalex": "W7223244301",
  "pdf_url": "https://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkag295/72372740/jkag295.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-22",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/ch5wrc84pkn71.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:09Z",
      "native_id": "10.1093/g3journal/jkag295",
      "source": "crossref",
      "url": "https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag295/8979769"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Genomics and Phylogenetic Studies",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A telomere-to-telomere (T2T) genome assembly of the Wuzhishan pig",
  "title_text": "A telomere-to-telomere (T2T) genome assembly of the Wuzhishan pig",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:09Z",
  "url": "https://research.draconitic.com/paper/ch5wrc84pkn71",
  "venue": "G3: Genes, Genomes, Genetics",
  "versions": []
}