{
  "abstract": "Native molecular neighborhoods govern biological processes and disease, yet existing approaches trade off penetration, precision, and native compatibility. Photocatalytic proximity labeling (PL) offers nanoscale, genetics-free precision but is limited by visible-light activation in optically opaque tissue. Here, we report 2PMap, a two-photon near-infrared PL platform driven by a sensitizer with a twophoton absorption cross-section nearly two orders of magnitude above conventional photocatalysts, enabling 850 nm continuous-wave excitation without pulsed lasers. In optically opaque samples, 2PMap achieved higher proteome coverage than conventional visible-light PL. In an early-stage Alzheimer’s disease mouse model, peptide-guided 2PMap produced the first proximity scale map of connective tissue growth factor (CTGF) in living brain tissue, capturing an 80-node network spanning extracellular-matrix, vascular, immune, and neuronal components within a microenvironment inaccessible to existing PL methods. 2PMap thus provides a genetics-free, modular platform for spatial proteomic mapping, extending molecular cartography to previously inaccessible living systems.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical Engineering，Guangdong Provincial Key Laboratory of Advanced Biomaterials",
    "Southern University of Science and Technology",
    "Shenzhen Key Laboratory of Gene Regulation and Systems Biology, and Brain Research Center, Department of Neuroscience, School of Life Sciences",
    "Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, The NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 18,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Jingkai Yang",
      "orcid": "0000-0001-8902-9237"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ziyu Wang"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Tao Deng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zimo Yao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ziyi Xu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zexi Tang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jiahui Lv"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Li Cheng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yanping Sun"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hongjun Xiao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jiaxin Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Guanghui Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Mingkun Lv"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Tong Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hancheng Song"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Kun Song",
      "orcid": "0000-0001-6216-8157"
    },
    {
      "affs": [
        3
      ],
      "name": "Pan-Lin Shao",
      "orcid": "0000-0001-7415-2195"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Bo Zhang",
      "orcid": "0000-0001-8745-2752"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/chna1czmez9bk.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15010771/v1",
  "fulltext_url": null,
  "id": "chna1czmez9bk",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010771/v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1307|Biotin and Related Studies"
    ]
  ],
  "openalex": "W7223093502",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-21",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/chna1czmez9bk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:24Z",
      "native_id": "10.26434/chemrxiv.15010771",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010771/v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Biotin and Related Studies",
      "subfield": "cell-biology"
    }
  ],
  "title": "Two-photon-enabled near-infrared proximity labeling maps protein neighborhoods in vivo",
  "title_text": "Two-photon-enabled near-infrared proximity labeling maps protein neighborhoods in vivo",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:24Z",
  "url": "https://research.draconitic.com/paper/chna1czmez9bk",
  "venue": "ChemRxiv",
  "versions": []
}