{
  "abstract": "Synthetic RNAs bearing deazapurine nucleobases are powerful probes for dissecting RNA-catalyzed reactions by atomic mutagenesis. Here we systematically characterize RNA containing 1-deazainosine (c1I) and compare it with inosine (I). We first report the synthesis of a suitably protected c1I phosphoramidite and its incorporation into RNA by solid-phase synthesis. We then provide a comprehensive thermodynamic analysis of base-pair stability from UV-melting experiments, showing that c1I–C pairs are less stable than the corresponding I–C pairs. Although a two-hydrogen-bond Hoogsteen interaction between c1I and protonated C is conceivable, NMR spectroscopy indicates that c1I–C predominantly adopts a Watson–Crick-like geometry with a single hydrogen bond. These pairs are accommodated within RNA duplexes without disrupting neighboring base pairing. We also use c1I to probe poly(I:C) motifs that mimic viral double-stranded RNA, assessing how strand length governs duplex versus hairpin formation. Finally, atomic mutagenesis of the twister ribozyme with c1I supports the hypothesis that an active-site guanine participates directly in phosphodiester-bond cleavage. Together, these results clarify how deazapurines modulate nucleic-acid properties and provide guidance for their use in atomic mutagenesis to interrogate RNA catalysis.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "Christoph Mitteregger"
    },
    {
      "name": "Raphael Bereiter"
    },
    {
      "name": "Antoine Schramm"
    },
    {
      "name": "Eric Ennifar"
    },
    {
      "name": "Christoph Kreutz"
    },
    {
      "name": "Ronald Micura"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/chxfz8wnezh0a.bib",
  "cited_by": 0,
  "doi": "10.1039/d6sc04009h",
  "fulltext_url": null,
  "id": "chxfz8wnezh0a",
  "issns": [
    "2041-6520",
    "2041-6539"
  ],
  "landing_url": "https://hal.science/hal-05772532v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.chim"
    ],
    [
      "openalex",
      "1312|RNA and protein synthesis mechanisms"
    ]
  ],
  "openalex": "W7163922727",
  "pdf_url": "https://hal.science/hal-05772532/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-06-08",
  "publisher": "The Royal Society of Chemistry",
  "ris": "https://research.draconitic.com/paper/chxfz8wnezh0a.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:44Z",
      "native_id": "hal-05772532",
      "source": "hal",
      "url": "https://hal.science/hal-05772532v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA and protein synthesis mechanisms",
      "subfield": "molecular-biology"
    }
  ],
  "title": "1-Deazainosine-impact on RNA structure and role in exploring ribozyme catalysis",
  "title_text": "1-Deazainosine-impact on RNA structure and role in exploring ribozyme catalysis",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:44Z",
  "url": "https://research.draconitic.com/paper/chxfz8wnezh0a",
  "venue": "Chemical Science",
  "versions": []
}