{
  "abstract": "Dual antiretroviral therapy with dolutegravir and lamivudine (DTG/3TC) is a recommended first-line regimen for people with HIV, but whether reduced-drug therapy maintains adequate antiviral pressure within lymph node (LN) reservoirs remains uncertain. In the DUALITY clinical trial (EudraCT number 2019-002733-10), the prespecified primary endpoint-change in proviral HIV-1 DNA in peripheral blood CD4 + T cells from baseline to week 48-was similar between participants initiating DTG/3TC or dolutegravir-based triple therapy. Here, we investigated prespecified secondary endpoints comprising viral persistence and antiretroviral drug penetration and distribution in LN using multimodal imaging. In 39 participants undergoing inguinal LN excision at baseline or during the first year of treatment, HIV-1 DNA and RNA were detected by DNAscope/RNAscope and antiretroviral spatial distribution was mapped by mass spectrometry imaging. HIV-1-infected cells declined rapidly after treatment initiation across both treatment groups. Antiretrovirals were widely detected within LN tissue regions containing or adjacent to HIV-positive cells. These findings provide spatial evidence that DTG/3TC reaches LN tissue and HIV-positive cells, extending peripheral blood observations to a key anatomical HIV reservoir.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 47,
  "authors": [
    {
      "name": "Elias P. Rosen",
      "orcid": "0000-0001-5531-7281"
    },
    {
      "name": "Stephen Bondoc"
    },
    {
      "name": "Lucía Bailón",
      "orcid": "0000-0001-7277-9314"
    },
    {
      "name": "Lidia Blay"
    },
    {
      "name": "Albert Caballero"
    },
    {
      "name": "Maria C. García-Guerrero"
    },
    {
      "name": "Maria C. Puertas",
      "orcid": "0000-0002-6750-2318"
    },
    {
      "name": "Igor Moraes-Cardoso",
      "orcid": "0000-0003-1011-8849"
    },
    {
      "name": "Andrea Vazquez-Escobales"
    },
    {
      "name": "Julià Blanco",
      "orcid": "0000-0002-2225-0217"
    },
    {
      "name": "Beatriz Mothe",
      "orcid": "0000-0001-9975-407X"
    },
    {
      "name": "Angela DM Kashuba"
    },
    {
      "name": "Jacob D. Estes",
      "orcid": "0000-0002-9652-6394"
    },
    {
      "name": "Javier Martinez-Picado",
      "orcid": "0000-0002-4916-2129"
    },
    {
      "name": "Jose Molto",
      "orcid": "0000-0003-4564-1963"
    },
    {
      "name": "On behalf of the DUALITY study group"
    },
    {
      "name": "Elias P. Rosen"
    },
    {
      "name": "Stephen Bondoc"
    },
    {
      "name": "Lucía Bailón"
    },
    {
      "name": "Lidia Blay"
    },
    {
      "name": "Albert Caballero"
    },
    {
      "name": "Maria C. García-Guerrero"
    },
    {
      "name": "Maria C. Puertas"
    },
    {
      "name": "Igor Moraes-Cardoso"
    },
    {
      "name": "Andrea Vazquez-Escobales"
    },
    {
      "name": "Julià Blanco"
    },
    {
      "name": "Beatriz Mothe"
    },
    {
      "name": "Angela DM Kashuba"
    },
    {
      "name": "Jacob D. Estes"
    },
    {
      "name": "Javier Martinez-Picado"
    },
    {
      "name": "Jose Molto"
    },
    {
      "name": "Joan Francesc Julian"
    },
    {
      "name": "Yovaninna Alarcón-Soto"
    },
    {
      "name": "Susana Benet"
    },
    {
      "name": "Cristina Miranda"
    },
    {
      "name": "Aroa Nieto"
    },
    {
      "name": "Roger Paredes"
    },
    {
      "name": "Angel Rivero"
    },
    {
      "name": "Sofia Sabato"
    },
    {
      "name": "Ester Aparicio"
    },
    {
      "name": "Christian Brander"
    },
    {
      "name": "Silvia Marfil"
    },
    {
      "name": "Alex Olvera"
    },
    {
      "name": "Edwards Pradenas"
    },
    {
      "name": "Victor Urrea"
    },
    {
      "name": "Yury Desyaterik"
    },
    {
      "name": "Nicole White"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cjb1dtzash712.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-78190-9",
  "fulltext_url": "https://europepmc.org/articles/PMC13724696",
  "id": "cjb1dtzash712",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://www.nature.com/articles/s41467-026-78190-9",
  "language": "eng",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2406|HIV Research and Treatment"
    ]
  ],
  "openalex": "W7216024381",
  "pdf_url": "https://www.nature.com/articles/s41467-026-78190-9_reference.pdf",
  "pmcid": "PMC13724696",
  "pmid": "42967902",
  "published": "2026-10-02",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/cjb1dtzash712.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:38Z",
      "native_id": "10.1038/s41467-026-78190-9",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41467-026-78190-9"
    },
    {
      "first_seen": "2026-11-03T15:20:53Z",
      "native_id": "MED:42967902",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42967902"
    },
    {
      "first_seen": "2026-11-04T12:23:13Z",
      "native_id": "PMC13724696",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41467-026-78190-9"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2406|HIV Research and Treatment",
      "subfield": "microbiology"
    }
  ],
  "title": "First-line dolutegravir/lamivudine in HIV-1 infection: lymph node penetration and reservoir decline in a randomized open-label clinical trial",
  "title_text": "First-line dolutegravir/lamivudine in HIV-1 infection: lymph node penetration and reservoir decline in a randomized open-label clinical trial",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:21:25Z",
  "url": "https://research.draconitic.com/paper/cjb1dtzash712",
  "venue": "Nature Communications",
  "versions": []
}