{
  "abstract": "Single-nucleus RNA sequencing (snRNA-seq) enables transcriptomic profiling of tissues that are difficult to dissociate. Here, we present an integrated protocol combining tissue-specific nuclei extraction and selective enrichment to isolate high-quality nuclei from fresh mouse liver and visceral adipose tissue (VAT). We describe steps for single-nucleus extraction, magnetic enrichment, BD Rhapsody capture, cDNA synthesis, whole-transcriptome amplification, index PCR, library quality control, and shallow sequencing-based assessment of cell-type recovery, while separating whole-liver and non-parenchymal cell workflows to reduce dissociation bias.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "Lei Li"
    },
    {
      "name": "Jonathan Pol"
    },
    {
      "name": "Christophe Desterke"
    },
    {
      "name": "Wanchao Hu"
    },
    {
      "name": "Nicolas Trainel"
    },
    {
      "name": "Morgane Benardeau"
    },
    {
      "name": "Manuela Lizarralde-Guerrero"
    },
    {
      "name": "Maria Chiara Maiuri"
    },
    {
      "name": "Guido Kroemer"
    },
    {
      "name": "Gabriel Perlemuter"
    },
    {
      "name": "Anne-Marie Cassard"
    },
    {
      "name": "Cosmin Sebastian Voican"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cmbec9e4pq0ne.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104699",
  "fulltext_url": null,
  "id": "cmbec9e4pq0ne",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://hal.science/hal-05793481v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|Single-cell and spatial transcriptomics"
    ]
  ],
  "openalex": "W7167691718",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-08",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/cmbec9e4pq0ne.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:54Z",
      "native_id": "hal-05793481",
      "source": "hal",
      "url": "https://hal.science/hal-05793481v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Single-cell and spatial transcriptomics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing",
  "title_text": "Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:54Z",
  "url": "https://research.draconitic.com/paper/cmbec9e4pq0ne",
  "venue": "STAR Protocols",
  "versions": []
}