{
  "abstract": "Background:: Foot and mouth disease (FMD) is ahighly contagious and economically burdensome viral disease of cloven-hoofed animals. Out of the seven FMD virus (FMDV) serotypes, type O is one of the most prevalent serotype in several parts of the world.\n\nAims:: This study aimed to develop a recombinant capsid polyprotein (rP1) and monoclonal antibody (MAb)-based solid phase competitive ELISA (rP1-MAb-SPCE) to detect anti-FMDV/O antibodies in serum samples.\n\nMethods:: For the development of rP1-MAb-SPCE the concentrations of rP1, MAb and dilution of serum were optimized. Percent inhibition (PI) cut-off was determined based on ROC curve analysis of cattle sera (n=53) of known FMD vaccination status with reference to the gold standard virus neutralization test (VNT). The assay performance was further validated in comparison to the conventional polyclonal antibody (PAb)-based SPCE using FMDV-positive and negative serum samples (n=612) obtained from multiple species (cattle, goat, pig, fallow deer, antelope, giraffe and African buffalo).\n\nResults:: At the selected cut-off value of 30.77 PI, the rP1-MAb-SPCE exhibited 86.21% diagnostic sensitivity and 95.83% diagnostic specificity. A significant correlation (r=0.84; P<0.0001) was observed between the PI values of sera tested by rP1-MAb-SPCE and PAb-based SPCE and also the limits of detection of both of the assays were comparable. Repeatability tests demonstrated that the coefficients of variation within and between runs were <10% and <14%, respectively.\n\nConclusion:: The developed rP1-MAb-SPCE has the potential to facilitate the detection of antibodies against FMDV serotype O while mitigating biosecurity concerns. However, further validation involving a larger serum cohort is essential to confirm the assay’s robustness.",
  "abstract_shown": true,
  "affiliations": [
    "ICAR-National Institute on Foot and Mouth Disease, Arugul, Bhubaneswar-752050, Odisha, India",
    "MSc in Biotechnology, ICAR-National Institute on Foot and Mouth Disease, Arugul, Bhubaneswar-752050, Odisha, India"
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "S. Mallick"
    },
    {
      "affs": [
        0
      ],
      "name": "R. P. Singh"
    },
    {
      "affs": [
        0
      ],
      "name": "J. K. Biswal"
    },
    {
      "affs": [
        0
      ],
      "name": "J. K. Mohapatra"
    },
    {
      "affs": [
        0
      ],
      "name": "M. Rout"
    },
    {
      "affs": [
        1
      ],
      "name": "S. K. Kabi"
    },
    {
      "affs": [
        0
      ],
      "name": "R. Ranjan"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cmttp4zcvmf48.bib",
  "cited_by": 0,
  "doi": "10.22099/ijvr.2026.53916.8090",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13734386/",
  "id": "cmttp4zcvmf48",
  "issns": [
    "1728-1997",
    "2252-0589"
  ],
  "landing_url": "https://doi.org/10.22099/ijvr.2026.53916.8090",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Short Paper"
    ],
    [
      "openalex",
      "1103|Animal Disease Management and Epidemiology"
    ]
  ],
  "openalex": "W7225347050",
  "pdf_url": null,
  "pmcid": "PMC13734386",
  "pmid": "42985678",
  "published": "2026-08-22",
  "publisher": "Shiraz University",
  "ris": "https://research.draconitic.com/paper/cmttp4zcvmf48.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:23:56Z",
      "native_id": "PMC13734386",
      "source": "pmc",
      "url": "https://doi.org/10.22099/ijvr.2026.53916.8090"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Animal Disease Management and Epidemiology",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "A developed recombinant antigen and monoclonal antibody based solid phase competitive ELISA for detection of foot and mouth disease virus serotype O specific antibodies",
  "title_text": "A developed recombinant antigen and monoclonal antibody based solid phase competitive ELISA for detection of foot and mouth disease virus serotype O specific antibodies",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:23:56Z",
  "url": "https://research.draconitic.com/paper/cmttp4zcvmf48",
  "venue": "Iranian Journal of Veterinary Research",
  "versions": []
}