{
  "abstract": "Background: Skin aging involves extracellular matrix (ECM) deterioration, including collagen loss and disrupted glycosaminoglycan and proteoglycan homeostasis. Pterostilbene (PTE) has antioxidant and anti-inflammatory activity, but its topical use is limited by poor aqueous solubility and limited skin delivery. We developed hyaluronic acid (HA)-modified, PTE-loaded nanoliposomes (HA-LP-PTE) and evaluated their association with dermal fibroblasts, retention in skin, and effects on ECM-related responses. Methods: HA-LP-PTE was prepared by high-pressure microfluidization and assessed for size, morphology, zeta potential, and encapsulation efficiency. Fluorescence microscopy was used to examine fibroblast-associated fluorescence of FITC-labeled HA-LP-PTE and non-HA-modified PTE-loaded liposomes (LP-PTE). Skin retention was assessed using Franz diffusion cells. ECM-related changes were analyzed in UV-irradiated ex vivo skin and fibroblasts using staining techniques and RT-qPCR. Results: HA-LP-PTE had a mean diameter of 55.44 ± 0.81 nm, a polydispersity index of 0.120 ± 0.009, and an encapsulation efficiency of 99.34 ± 0.51%. At 4 h, fibroblast-associated fluorescence was higher than with LP-PTE (p = 0.040) and was reduced by free HA or incubation at 4 °C. After 12 h, dermal PTE retention was 4.08-fold higher than free PTE and 1.74-fold higher than LP-PTE. HA-LP-PTE increased all six ECM components examined. Chondroitin sulfate and perlecan met the Q criterion for synergy, and several ECM synthesis-related genes were upregulated. Conclusions: HA modification improved PTE delivery beyond liposomal encapsulation alone, with greater fibroblast-associated fluorescence and higher PTE retention in viable skin than LP-PTE. ECM and gene-expression findings also indicated matrix-related activity. HA-LP-PTE may enhance topical PTE delivery and attenuate UV-induced ECM loss.",
  "abstract_shown": true,
  "affiliations": [
    "Meyer Bio-Medicine Co., Ltd., Jinan 250022, China.",
    "Shandong Meimao Pharmaceutical Co., Ltd., Jinan 250022, China.",
    "National Glycoengineering Research Center, Shandong University, Jinan 250100, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Zheng Wang",
      "orcid": "0009-0008-2991-1509"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yumei Fan",
      "orcid": "0000-0001-5238-1106"
    },
    {
      "affs": [
        2
      ],
      "name": "Peixue Ling"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cnfxpttdnndn2.bib",
  "cited_by": 0,
  "doi": "10.3390/pharmaceutics18101313",
  "fulltext_url": "https://europepmc.org/articles/PMC13708579",
  "id": "cnfxpttdnndn2",
  "issns": [
    "1999-4923"
  ],
  "landing_url": "https://europepmc.org/article/MED/42942843",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "3003|Advancements in Transdermal Drug Delivery"
    ]
  ],
  "openalex": "W7222760108",
  "pdf_url": "https://europepmc.org/articles/PMC13708579?pdf=render",
  "pmcid": "PMC13708579",
  "pmid": "42942843",
  "published": "2026-10-16",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cnfxpttdnndn2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42942843",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42942843"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:3003|Advancements in Transdermal Drug Delivery",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Multifunctional Hyaluronic Acid-Modified Pterostilbene Nanoliposomes for Enhanced Dermal Delivery and Extracellular Matrix Protection.",
  "title_text": "Multifunctional Hyaluronic Acid-Modified Pterostilbene Nanoliposomes for Enhanced Dermal Delivery and Extracellular Matrix Protection.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/cnfxpttdnndn2",
  "venue": "Pharmaceutics",
  "versions": []
}