{
  "abstract": "Nicotinic acetylcholinergic receptors (nAChRs), comprising of α and β subunits are present in the brain and whole body. The less abundant α2-subunit is a fast-acting receptor subtype and plays an important role in cognition and learning. To understand cellular functional consequences, this study evaluated glucose metabolism using [18F]FDG PET/CT in α2 knockout (α2KO) and α2 hypersensitive (α2HS) mice. Control (CN; 4M, 3F), α2 knockout (α2KO; 4M, 4F) and α2 hypersensitive (α2HS; 4M,4F), 12–16 month old mice were used. Mice were fasted and injected with [18F]FDG (3–5 MBq) while awake. After 40 minutes they underwent whole body PET/CT. On a separate day, nicotine challenge [18F]FDG studies were done. Reconstructed images were analyzed to obtain standard uptake values (SUV) of [18F]FDG in brain and interscapular brown adipose tissue (IBAT). Statistical analysis was performed comparing the three groups of mice. The α2HS male mice exhibited the largest brain increase in [18F]FDG compared to α2KO male mice. The rank order of brain [18F]FDG uptake in the three groups: α2HS♂ > CN♂ > α2KO♂ > CN♀= = α2KO♀ ≥ α2HS♀. Nicotine treatment reduced brain [18F]FDG uptake in all mice. Females had lower [18F]FDG uptake compared to males and were less sensitive to α2 nAChR. In the case of IBAT, α2KO mice had significantly higher baseline [18F]FDG uptake compared to the other two groups: α2KO♂ > α2KO♀ > α2HS♀ > α2HS♂ > CN♀ > CN♂. Nicotine decreased IBAT in α2KO mice rather than increase as observed in CN and α2HS mice.This preliminary study shows that α2 nAChRs play a significant role in brain activation as exhibited by the increase in [18F]FDG in α2HS mice. In the absence of regulatory control by the α2 nAChR, the α2KO mice IBAT exhibited higher [18F]FDG uptake compared to controls and α2HS mice. Female mice were less affected by nicotine compared to the male mice.",
  "abstract_shown": true,
  "affiliations": [
    "Preclinical Imaging, Department of Radiological Sciences, University of California, Irvine, CA, United States of America",
    "Department of Neurobiology and Behavior, University of California, Irvine, CA, United States of America"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Christopher Liang",
      "orcid": "0000-0001-9081-294X"
    },
    {
      "affs": [
        0
      ],
      "name": "Titus E. Tucker"
    },
    {
      "affs": [
        0
      ],
      "name": "Ashlee D. L. Coronel",
      "orcid": "0009-0003-2810-766X"
    },
    {
      "affs": [
        0
      ],
      "name": "Ellen H. N. Nguyen"
    },
    {
      "affs": [
        0
      ],
      "name": "Janet L. Nguyen",
      "orcid": "0009-0006-1295-2238"
    },
    {
      "affs": [
        1
      ],
      "name": "Irakli Intskirveli"
    },
    {
      "affs": [
        1
      ],
      "name": "Ronit Lazar"
    },
    {
      "affs": [
        1
      ],
      "name": "Raju Metherate"
    },
    {
      "affs": [
        0
      ],
      "name": "Jogeshwar Mukherjee",
      "orcid": "0000-0003-1009-877X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cp8720ztprrjh.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0346469",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13712757/",
  "id": "cp8720ztprrjh",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0346469",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Acetylcholine Receptors"
    ],
    [
      "heading",
      "Addiction"
    ],
    [
      "heading",
      "Adipose Tissue"
    ],
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      "heading",
      "Alkaloids"
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      "heading",
      "Amniotes"
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      "heading",
      "Anatomy"
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    [
      "heading",
      "Biological Tissue"
    ],
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      "heading",
      "Biology and Life Sciences"
    ],
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      "heading",
      "Brown Adipose Tissue"
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    [
      "heading",
      "Carbohydrate Metabolism"
    ],
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      "heading",
      "Cell Biology"
    ],
    [
      "heading",
      "Chemical Compounds"
    ],
    [
      "heading",
      "Chemistry"
    ],
    [
      "heading",
      "Connective Tissue"
    ],
    [
      "heading",
      "Diagnostic Medicine"
    ],
    [
      "heading",
      "Diagnostic Radiology"
    ],
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      "heading",
      "Drug Therapy"
    ],
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      "heading",
      "Eukaryota"
    ],
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      "heading",
      "Glucose Metabolism"
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      "heading",
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    ],
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      "heading",
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    ],
    [
      "heading",
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    ],
    [
      "heading",
      "Nicotine"
    ],
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      "heading",
      "Nicotine Addiction"
    ],
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      "heading",
      "Nicotine Replacement Therapy"
    ],
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      "heading",
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      "heading",
      "Public and Occupational Health"
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      "heading",
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      "heading",
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      "heading",
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      "heading",
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      "heading",
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    [
      "heading",
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      "openalex",
      "1312|Nicotinic Acetylcholine Receptors Study"
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    [
      "plos",
      "/Biology and life sciences/Anatomy/Biological tissue/Connective tissue/Adipose tissue/Brown adipose tissue"
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      "plos",
      "/Biology and life sciences/Biochemistry/Metabolism/Carbohydrate metabolism/Glucose metabolism"
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      "/Medicine and health sciences/Radiology and imaging/Diagnostic radiology/Tomography/Positron emission tomography"
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      "/Physical sciences/Chemistry/Chemical compounds/Alkaloids/Nicotine"
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    [
      "plos",
      "/Research and analysis methods/Imaging techniques/Neuroimaging/Positron emission tomography"
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  "pmcid": "PMC13712757",
  "pmid": "42945313",
  "published": "2026-10-27",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/cp8720ztprrjh.ris",
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    {
      "first_seen": "2026-10-30T12:17:17Z",
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  "subjects": [
    {
      "field": "biology",
      "origin": "heading:Cell Biology",
      "subfield": "cell-biology"
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    {
      "field": "chemistry",
      "origin": "heading:Chemistry",
      "subfield": "general"
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    {
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      "origin": "plos:anatomy",
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      "field": "medicine",
      "origin": "heading:Mental Health and Psychiatry",
      "subfield": "psychiatry"
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  "title": "Imaging [¹⁸F]FDG PET/CT pilot study of nicotinic acetylcholinergic receptor α2 knock-out mice and α2 hypersensitive mice compared to control mice: Male-female differences and nicotine effects",
  "title_text": "Imaging [¹⁸F]FDG PET/CT pilot study of nicotinic acetylcholinergic receptor α2 knock-out mice and α2 hypersensitive mice compared to control mice: Male-female differences and nicotine effects",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:23:16Z",
  "url": "https://research.draconitic.com/paper/cp8720ztprrjh",
  "venue": "PLOS One",
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}