{
  "abstract": "Bombyx mori latent virus (BmLV) is a persistent RNA virus frequently detected in silkworm-derived cell lines and has been reported to interact with B. mori nucleopolyhedrovirus (BmNPV). However, whether previous BmLV exposure alters subsequent BmNPV infection in silkworm larvae remains unclear. Here, we examined how BmLV exposure history affects subsequent BmNPV infection in cultured cells and fifth-instar B. mori larvae. Persistent BmLV infection increased BmNPV-mediated reporter protein expression and BmNPV genome abundance in cultured cells, whereas short-term BmLV exposures, including simultaneous exposure to BmLV and BmNPV, did not reproduce these effects. In contrast, 24 h BmLV pre-exposure before BmNPV challenge increased BmNPV-mediated reporter protein expression and BmNPV genome abundance in f38 and n22 silkworm larvae. BmLV antigenome RNA was detected in multiple larval tissues after BmLV exposure, indicating BmLV replication during the pre-exposure period. Notably, following subsequent BmNPV infection, BmLV-pre-exposed larvae showed reduced induction of Dicer-2, R2D2, and stimulator of interferon genes (STING) in the fat body. These findings indicate that prior BmLV exposure enhances subsequent BmNPV infection and is associated with attenuated induction of host antiviral genes. This study expands our understanding of how sequential viral infections shape host-virus interactions and suggests that BmLV exposure history may influence baculovirus-based recombinant protein expression.",
  "abstract_shown": true,
  "affiliations": [
    "Kyushu University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "N. Abubakar"
    },
    {
      "name": "H. Mon"
    },
    {
      "name": "T. Fujii"
    },
    {
      "name": "T. Fujimoto"
    },
    {
      "name": "T. Kusakabe"
    },
    {
      "affs": [
        0
      ],
      "name": "M. Hino"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/crkg92jfmbc9t.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.14.758696",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.14.758696v1.full",
  "id": "crkg92jfmbc9t",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.14.758696v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "2403|Invertebrate Immune Response Mechanisms"
    ]
  ],
  "openalex": "W7222105252",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.14.758696v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/crkg92jfmbc9t.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:28:01Z",
      "native_id": "10.64898/2026.10.14.758696",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.14.758696v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Prior Bombyx mori latent virus exposure enhances subsequent BmNPV infection and suppresses antiviral responses in the silkworm, Bombyx mori",
  "title_text": "Prior Bombyx mori latent virus exposure enhances subsequent BmNPV infection and suppresses antiviral responses in the silkworm, Bombyx mori",
  "type": "preprint",
  "updated_at": "2026-10-30T12:30:16Z",
  "url": "https://research.draconitic.com/paper/crkg92jfmbc9t",
  "venue": "bioRxiv",
  "versions": []
}