{
  "abstract": "Ischemic stroke triggers profound, dynamic changes in the brain's cellular landscape. To systematically characterize these dynamics, we integrated single-cell RNA-sequencing data from 21 mouse stroke studies (103 samples; 414,359 cells) spanning multiple models and post-stroke intervals. Using a harmonized computational workflow, we constructed StrokeTime, a single-cell mouse brain atlas covering hyperacute, acute, subacute, and chronic stages post-ischemia. The atlas contains 17 major cell classes and 120 fine-grained transcriptional states within myeloid, lymphoid, vascular, epithelial, neuronal, and glial populations. Myeloid cells showed the greatest plasticity, including disease-associated microglia/macrophage programs shared across resident and infiltrating cells, whereas lymphocytes featured activation and regulatory signatures within lineage constraints. Vascular and mural cells shifted from early inflammatory and barrier-disruptive programs to later fibrotic and angiogenic states. StrokeTime delineates both convergent and cell type-specific injury responses, offering a resource for studying post-ischemic brain immunity, tissue remodeling, and benchmarking future single-cell and single-nucleus studies of cerebrovascular disease.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "name": "Josef Anrather"
    },
    {
      "name": "Lidia Garcia-Bonilla",
      "orcid": "0000-0003-3832-0037"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/csqb4m3k6w3h7.bib",
  "cited_by": 0,
  "doi": "10.1016/j.isci.2026.117938",
  "fulltext_url": "https://europepmc.org/articles/PMC13695117",
  "id": "csqb4m3k6w3h7",
  "issns": [
    "2589-0042"
  ],
  "landing_url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03321-3",
  "language": "eng",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "http://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Biological sciences"
    ],
    [
      "heading",
      "Genomics"
    ],
    [
      "heading",
      "Neuroscience"
    ],
    [
      "heading",
      "Transcriptomics"
    ],
    [
      "openalex",
      "1312|Single-cell and spatial transcriptomics"
    ]
  ],
  "openalex": "W7222980617",
  "pdf_url": "https://europepmc.org/articles/PMC13695117?pdf=render",
  "pmcid": "PMC13695117",
  "pmid": "42927268",
  "published": "2026-10-19",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/csqb4m3k6w3h7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:16Z",
      "native_id": "10.1016/j.isci.2026.117938",
      "source": "feed-cell",
      "url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03321-3"
    },
    {
      "first_seen": "2026-10-30T12:17:49Z",
      "native_id": "10.1016/j.isci.2026.117938",
      "source": "crossref",
      "url": "https://linkinghub.elsevier.com/retrieve/pii/S2589004226033213"
    },
    {
      "first_seen": "2026-10-30T12:21:18Z",
      "native_id": "MED:42927268",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42927268"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "heading:Genomics",
      "subfield": "genetics-genomics"
    },
    {
      "field": "biology",
      "origin": "heading:Neuroscience",
      "subfield": "neuroscience"
    }
  ],
  "title": "StrokeTime: A comprehensive longitudinal scRNA-seq atlas of the ischemic mouse brain",
  "title_text": "StrokeTime: A comprehensive longitudinal scRNA-seq atlas of the ischemic mouse brain",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:18Z",
  "url": "https://research.draconitic.com/paper/csqb4m3k6w3h7",
  "venue": "iScience",
  "versions": []
}