{
  "abstract": "Background/Objectives: Osteoporosis is a progressive metabolic bone disorder with reduced bone mass and microarchitectural deterioration, leading to an increased risk of fractures. With an aging population, there is a growing demand for safe and effective long-term therapeutic methods for osteoporosis and medicinal plants could be promising candidates as novel agents for osteoporosis. In this study, we investigated the effects of the 70% ethanol extract of Quercus salicina Blume (QS), an herbal plant, on osteoporosis. Methods: MC3T3-E1 mouse pre-osteoblast cells and RAW264.7 mouse macrophage cells were utilized to assess osteoblast and osteoclast differentiation, respectively. QSEE (10 mg/kg and 40 mg/kg) was orally administered for 12 weeks in OVX mice, a postmenopausal osteoporosis animal model, and bone parameters were evaluated using Micro-Computed Tomography (Micro-CT). Ex vivo experiments were conducted using primary cells isolated from mouse femurs and tibias. Expression levels of proteins and mRNAs related to osteoblasts and osteoclasts were estimated by Western blot and Quantitative Real-Time PCR (qRT-PCR). Results: QSEE showed osteogenic activity by increasing alkaline phosphatase (ALP) activity, protein and mRNA expression related to osteoblast differentiation. QSEE decreased osteoclast differentiation by suppressing tartrate-resistant acid phosphatase (TRAP) activity, protein and mRNA expression related to osteoclast differentiation. In OVX mice, QSEE administration restored bone microarchitecture decreased by OVX. In primary cells isolated from mouse femurs, QSEE increased osteoblast-related markers and decreased osteoclast-related markers. Conclusions: These findings suggest Quercus salicina Blume has potential as an anti-osteoporosis agent by inducing osteoblast differentiation and suppressing osteoclast differentiation.",
  "abstract_shown": true,
  "affiliations": [
    "Gangneung Science & Industrial Promotion Agency, Gangneung 25440, Republic of Korea",
    "Smart Farm Research Center, Gangneung Institute of Natural Products, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of Korea",
    "Division of Natural Product Applied Science, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of Korea",
    "Department of Plant Science, Kangwon National University, Gangneung 25457, Republic of Korea"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Suhyun Cho"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Nadzeya Lazerka",
      "orcid": "0009-0000-7974-6072"
    },
    {
      "affs": [
        1
      ],
      "name": "Soyeon Hong"
    },
    {
      "affs": [
        1
      ],
      "name": "Minji Gwon"
    },
    {
      "affs": [
        3
      ],
      "name": "Byeong Jun Jeon"
    },
    {
      "affs": [
        1
      ],
      "name": "Jeong Do Kim",
      "orcid": "0000-0001-6388-522X"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Gyhye Yoo",
      "orcid": "0000-0002-3033-3178"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ctfgrhkdyfc1d.bib",
  "cited_by": 0,
  "doi": "10.3390/nu18213578",
  "fulltext_url": null,
  "id": "ctfgrhkdyfc1d",
  "issns": [
    "2072-6643"
  ],
  "landing_url": "https://www.mdpi.com/2072-6643/18/21/3578",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-29",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/ctfgrhkdyfc1d.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:56Z",
      "native_id": "10.3390/nu18213578",
      "source": "crossref",
      "url": "https://www.mdpi.com/2072-6643/18/21/3578"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "issn:2072-6643",
      "subfield": "nutrition"
    }
  ],
  "title": "Quercus salicina Blume Inhibits Bone Loss via Dual Effects on Osteoblasts and Osteoclasts",
  "title_text": "Quercus salicina Blume Inhibits Bone Loss via Dual Effects on Osteoblasts and Osteoclasts",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T00:21:06Z",
  "url": "https://research.draconitic.com/paper/ctfgrhkdyfc1d",
  "venue": "Nutrients",
  "versions": []
}