{
  "abstract": "Background/purpose\n\nOral squamous cell carcinoma (OSCC) remains a global health issue, particularly in underserved settings such Taiwan where access to early diagnosis is limited. DNA ploidy-based oral cytology has shown strong diagnostic performance, but its applicability across populations require evaluation.Materials and methods\n\nIn this prospective feasibility study, brush and oral rinse samples were collected from patients with OSCC, low-grade dysplasia, reactive lesions, and normal mucosa. DNA cytometry was performed to quantify ploidy profiles. Diagnostic performance was evaluated using published algorithm and cohort-specific thresholds derived from stratified 10-fold cross-validation.Results\n\nA total of 118 patients were included (50 OSCC, 41 normal, 22 reactive lesions, and 5 low-grade dysplasia). Applying the published algorithm to brushing samples yielded high sensitivity (0.97), but much poor specificity (0.27), highlighting cohort-specific limitations. New thresholds were therefore derived using 10-fold cross-validation based on the percentage of abnormal cells. Among 82 adequate brush samples, a threshold of 3.7% abnormal cells achieved 0.75 sensitivity 0.75 and 0.86 specificity. For 95 adequate rinse samples, a 1.4% threshold yielded 0.77 sensitivity and 0.80 specificity. Thresholds based on non-diploid cells performed worse. Covariate-adjusted logistic regression further improved sensitivity to 0.94 (brush) and 0.87 (rinse). In 105 paired samples, rinse testing demonstrated fewer inadequate samples (12.5% vs. 22.5%) and higher cell yield.Conclusion\n\nThis is the first study assessing automated DNA cytology in an areca nut-prevalent population. Findings emphasize the need for population-specific calibration and support rinse-based cytology as a scalable self-administered screening approach. Large multicenter validation is warranted.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Dentistry, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan.",
    "Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.",
    "Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.",
    "Department of Oral Biological and Medical Sciences, Faculty of Dentistry, The University of British Columbia, Vancouver, BC, Canada.",
    "Basic and Translational Research, BC Cancer Research Institute, Vancouver, BC, Canada.",
    "School of Biomedical Engineering, Faculty of Medicine, The University of British Columbia, Vancouver, BC, Canada.",
    "Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.",
    "Department of Pathology, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan.",
    "Department of Oncology, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Pei Hsuan Lu"
    },
    {
      "affs": [
        3,
        4,
        5
      ],
      "name": "Kelly Yi Ping Liu"
    },
    {
      "affs": [
        1,
        2,
        6
      ],
      "name": "Julia Yu Fong Chang"
    },
    {
      "affs": [
        7
      ],
      "name": "Long-Wei Lin"
    },
    {
      "affs": [
        1
      ],
      "name": "Hao-Hung Chang"
    },
    {
      "affs": [
        8
      ],
      "name": "Chen-Han Chang"
    },
    {
      "affs": [
        8
      ],
      "name": "Shang Hsuan Peng"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Catherine F Poh"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cvh8g0bvkn01k.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jds.2025.07.3578",
  "fulltext_url": "https://europepmc.org/articles/PMC13689659",
  "id": "cvh8g0bvkn01k",
  "issns": [
    "1991-7902",
    "2213-8862"
  ],
  "landing_url": "https://europepmc.org/article/MED/42924204",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2733|Head and Neck Cancer Studies"
    ]
  ],
  "openalex": "W7223578013",
  "pdf_url": "https://europepmc.org/articles/PMC13689659?pdf=render",
  "pmcid": "PMC13689659",
  "pmid": "42924204",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cvh8g0bvkn01k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42924204",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42924204"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2733|Head and Neck Cancer Studies",
      "subfield": "general"
    }
  ],
  "title": "Developing an automated cytology-based DNA ploidy test for detecting oral squamous cell carcinoma in Taiwan: A feasibility study.",
  "title_text": "Developing an automated cytology-based DNA ploidy test for detecting oral squamous cell carcinoma in Taiwan: A feasibility study.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/cvh8g0bvkn01k",
  "venue": "Journal of dental sciences",
  "versions": []
}