{
  "abstract": "ObjectivesThe near point-of-care (nPOC) Pluslife MiniDock MTB (MiniDock) assay does not report semiquantitative values. We evaluated whether categorized MiniDock time-to-positivity (TTP) serves as a quantitative proxy for Mycobacterium tuberculosis (Mtb) bacterial load.\n\nMethodsPresumptive tuberculosis (TB) patients enrolled across 27 health facilities in Indonesia were tested with sputum GeneXpert MTB/RIF Ultra (Xpert), MiniDock sputum swabs, and tongue swabs. Positive results were categorized using a median split at 13 minutes ([≤]13, 13-25, and 25 minute). MiniDock TTP categories were evaluated against Xpert semiquantitative grades and BACTEC MGIT 960 liquid culture TTP (days).\n\nResultsOf 2974 presumptive TB participants tested with sputum Xpert, 426 (14.3%) were sputum Xpert-positive. MiniDock detected Mtb in 248/299 (83.0%) sputum and 263/382 (68.8%) tongue swab. Among 248 Minidock sputum-positive results, 99 (39.9%) turned positive [≤]13 minutes, 107 (43.1%) between 13 and 25 minutes, and 42 (16.9%) at 25 minutes. Minidock TTP categories correlated with sputum and tongue swab semiquantitative results as well as with culture time to positivity (p<0.001).\n\nConclusionsMiniDock TTP categories ([≤]13, 13-25, 25 minutes) provide meaningful stratification which correlates with both Xpert semiquantitative and culture TTP. Time to Positivity from nPOC could thus serve as a proxy for bacterial burden and infectiousness, strongly increasing its utility for clinical care, public health and research.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Internal Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands"
  ],
  "article_type": "PUBLISHAHEADOFPRINT",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "M. Korompis"
    },
    {
      "name": "L. D. Veeken"
    },
    {
      "name": "S. Hartati"
    },
    {
      "name": "Z. H. Fatma"
    },
    {
      "name": "L. Chaidir"
    },
    {
      "name": "N. Eristiana"
    },
    {
      "name": "T. Setiabudiawan"
    },
    {
      "name": "J. van Ingen"
    },
    {
      "name": "R. van Crevel"
    },
    {
      "name": "P. C. Hill"
    },
    {
      "name": "R. M. G. J. Houben"
    },
    {
      "name": "B. Alisjahbana"
    },
    {
      "name": "R. C. Koesoemadinata"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cvqgeywkben1g.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.01.26361863",
  "fulltext_url": "https://www.medrxiv.org/content/10.64898/2026.09.01.26361863v1.full",
  "id": "cvqgeywkben1g",
  "issns": [],
  "landing_url": "https://www.medrxiv.org/content/10.64898/2026.09.01.26361863v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "medrxiv",
      "infectious diseases"
    ],
    [
      "openalex",
      "2725|Tuberculosis Research and Epidemiology"
    ]
  ],
  "openalex": "W7208778483",
  "pdf_url": "https://www.medrxiv.org/content/10.64898/2026.09.01.26361863v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cvqgeywkben1g.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:47Z",
      "native_id": "10.64898/2026.09.01.26361863",
      "source": "medrxiv",
      "url": "https://www.medrxiv.org/content/10.64898/2026.09.01.26361863v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "medrxiv:infectious diseases",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Association of Pluslife MiniDock MTB time-to-positivity with tuberculosis bacterial load: a diagnostic study in Indonesia",
  "title_text": "Association of Pluslife MiniDock MTB time-to-positivity with tuberculosis bacterial load: a diagnostic study in Indonesia",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:47Z",
  "url": "https://research.draconitic.com/paper/cvqgeywkben1g",
  "venue": "medRxiv",
  "versions": []
}