{
  "abstract": "Bovine viral diarrhea virus (BVDV) is a widespread pathogen causing significant economic losses in cattle. There is an urgent need for drugs to treat BVDV-induced reproductive dysfunction. This study investigated the mechanisms and targets of the cryptotanshinone-curcumin (CRY-CUR) combination against BVDV-1. Overlapping targets of CRY, CUR, and BVDV were screened using TCMSP, ChEMBL, and GeneCards databases. A Venn diagram was generated, followed by GO and KEGG enrichment analyses via DAVID. A PPI network was constructed using Cytoscape, and molecular docking was performed with AutoDock. Anti-BVDV-1 activity was verified in bovine ovarian granulosa cells (BOGCs) using RT-qPCR and immunofluorescence. A total of 108 overlapping targets were identified, with four core targets (STAT3, TNF, BCL-2, and EGFR) selected. Molecular docking showed strong binding affinities. Enrichment analysis indicated involvement in calcium ion binding, apoptosis, and TNF/NF-κB pathways. In vitro, the CRY-CUR combination suppressed BVDV-1 replication and reversed abnormal mRNA expression of the core targets. The CRY-CUR combination may inhibit BVDV-1 replication, potentially through modulation of BVDV-1-related targets such as STAT3, TNF, BCL-2, and EGFR and their associated signaling pathways.",
  "abstract_shown": true,
  "affiliations": [
    "Hebei Key Laboratory of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071000, China.",
    "Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.",
    "College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xiaoliang Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Kang Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Haipeng Feng"
    },
    {
      "affs": [
        1
      ],
      "name": "Lei Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Jingyan Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaorong Lu"
    },
    {
      "affs": [
        1
      ],
      "name": "Guowei Xu"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Siqi Liu"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaowei Feng"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Junyan Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jianxi Li"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cvwxwyqdqgkrd.bib",
  "cited_by": 0,
  "doi": "10.3390/vetsci13101002",
  "fulltext_url": "https://europepmc.org/articles/PMC13709452",
  "id": "cvwxwyqdqgkrd",
  "issns": [
    "2306-7381"
  ],
  "landing_url": "https://europepmc.org/article/MED/42944098",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1103|Animal Virus Infections Studies"
    ]
  ],
  "openalex": "W7214090855",
  "pdf_url": "https://europepmc.org/articles/PMC13709452?pdf=render",
  "pmcid": "PMC13709452",
  "pmid": "42944098",
  "published": "2026-09-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cvwxwyqdqgkrd.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42944098",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42944098"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Animal Virus Infections Studies",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Mechanistic Investigation of Cryptotanshinone and Curcumin Combination in Attenuating BVDV-1-Induced Injury in Bovine Ovarian Granulosa Cells.",
  "title_text": "Mechanistic Investigation of Cryptotanshinone and Curcumin Combination in Attenuating BVDV-1-Induced Injury in Bovine Ovarian Granulosa Cells.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/cvwxwyqdqgkrd",
  "venue": "Veterinary sciences",
  "versions": []
}