{
  "abstract": "Alternative lengthening of telomeres (ALT) is a telomerase-independent, recombination-based pathway utilized by some cancers. Previously, we established 2 distinct types of ALT survivors derived from mouse embryonic stem cells (mESCs), characterized by either a unique non-telomeric insertion (type I) or canonical telomeric repeats (type II). In this study, we used telomere-specific proteomics to elucidate the divergent telomere maintenance strategies of these ALT types. We identified distinct proteomic profiles enriched in type I versus type II ALT telomeres. ICE1 is a component of the Little Elongation Complex, which is specifically recruited to type I ALT telomeres, where it supports the transcription of telomeric repeat-containing RNA and telomere maintenance. In contrast, the serine/threonine kinase VRK2 is highly enriched in type II ALT telomeres, where it regulates telomere–nuclear envelope interactions. Depletion of ICE1 or VRK2 impairs telomere stability in type I and type II ALT mESCs, respectively. Our results indicate that cells with a common mESC origin can adopt distinct molecular mechanisms to maintain telomere integrity, highlighting the mechanistic heterogeneity of ALT telomere maintenance.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biological Sciences, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 08826, Korea",
    "Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Korea",
    "Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA",
    "Institute of Human Genetics, CNRS-University of Montpellier UMR 9002, 141 Cardonille Street, Montpellier 34000, France",
    "Department of Biomedical Engineering, College of Information and Biotechnology, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulju-gun, Ulsan 44919, Korea",
    "Aging Convergence Research Center (ACRC), Development and Differentiation Research Center, Korea Research Institute of Bioscience Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, South Korea",
    "Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA",
    "Institute Genomics Functional, CNRS-University of Montpellier UMR 5203, 141 Cardonille Street, Montpellier 34000, France",
    "Hamamatsu University School of Medicine, 1-20-1 Handayama, Chuo-ku, Hamamatsu city, Shizuoka 431-3192, Japan",
    "Research Institute of Basic Sciences, Seoul National University, Seoul 08826, Korea"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Hyunji Lee"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Kyumin Park"
    },
    {
      "affs": [
        3
      ],
      "name": "Sandrine Sauzet"
    },
    {
      "affs": [
        4
      ],
      "name": "Subin Choi"
    },
    {
      "affs": [
        4
      ],
      "name": "Seung Woo Cho"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Christine Hyein Chung"
    },
    {
      "affs": [
        5
      ],
      "name": "Sanghyun Sung"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Dongan Kwon"
    },
    {
      "affs": [
        0,
        6
      ],
      "name": "Hobum Song"
    },
    {
      "affs": [
        7
      ],
      "name": "Serge Urbach"
    },
    {
      "affs": [
        8
      ],
      "name": "Hiroyuki Niida"
    },
    {
      "affs": [
        3
      ],
      "name": "Jérôme Déjardin"
    },
    {
      "affs": [
        0,
        1,
        9
      ],
      "name": "Junho Lee"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cw1kpefn57eex.bib",
  "cited_by": 0,
  "doi": "10.1016/j.mocell.2026.100405",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13738406/",
  "id": "cw1kpefn57eex",
  "issns": [
    "1016-8478",
    "0219-1032"
  ],
  "landing_url": "https://doi.org/10.1016/j.mocell.2026.100405",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Fast-Track"
    ],
    [
      "openalex",
      "1307|Telomeres, Telomerase, and Senescence"
    ]
  ],
  "openalex": "W7220897962",
  "pdf_url": "https://www.sciencedirect.com/science/article/pii/S1016847826000968/pdf",
  "pmcid": "PMC13738406",
  "pmid": "42849576",
  "published": "2026-10-08",
  "publisher": "Korean Society for Molecular and Cellular Biology",
  "ris": "https://research.draconitic.com/paper/cw1kpefn57eex.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:23:23Z",
      "native_id": "PMC13738406",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.mocell.2026.100405"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Telomeres, Telomerase, and Senescence",
      "subfield": "cell-biology"
    }
  ],
  "title": "Divergent proteomic landscapes and functional requirements for telomere maintenance in type I and type II alternative lengthening of telomeres in mouse embryonic stem cells",
  "title_text": "Divergent proteomic landscapes and functional requirements for telomere maintenance in type I and type II alternative lengthening of telomeres in mouse embryonic stem cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:23:23Z",
  "url": "https://research.draconitic.com/paper/cw1kpefn57eex",
  "venue": "Molecules and Cells",
  "versions": []
}