{
  "abstract": "Human adipose tissue models are lacking although essential for metabolic research. Traditional primary cell cultures are poorly scalable and unsuitable for drug screening or modeling of genetic disease. Conventional two-dimensional adipocyte cultures cannot withstand the long-term treatment required to mimic chronic conditions such as obesity and insulin resistance. To overcome these limitations, we developed an accessible protocol to produce three-dimensional human induced pluripotent stem cell (hiPSC)-derived adipocyte microtissues, termed adipoids. hiPSC-derived adipoids display long-term stability and viability. Single-nucleus RNA sequencing revealed that adipoids contain mature adipocytes and adipocyte stem/progenitor cells, similar to human adipose tissue. Furthermore, hiPSC-derived adipoids contain unilocular adipocytes, exhibit stimulated lipolysis, and secrete adipokines. Thus, hiPSC-derived adipoids provide a functional, scalable and genetically tractable model to perform large-scale pharmacological screens and to investigate human metabolic disease.",
  "abstract_shown": true,
  "affiliations": [
    "Biozentrum, University of Basel Basel, Switzerland"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "name": "L. C. Hinte"
    },
    {
      "name": "R. Okamoto"
    },
    {
      "name": "S. Jansen"
    },
    {
      "name": "S. E. Spirig"
    },
    {
      "name": "S. Tsiepa"
    },
    {
      "name": "M. Shimobayashi"
    },
    {
      "affs": [
        0
      ],
      "name": "M. N. Hall"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cwaj61bsj6zqh.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.30.762496",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.762496v1.full",
  "id": "cwaj61bsj6zqh",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.762496v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "molecular biology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.762496v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cwaj61bsj6zqh.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T00:20:41Z",
      "native_id": "10.64898/2026.10.30.762496",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.30.762496v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:molecular biology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Generation of functional adipoids from human induced pluripotent stem cells",
  "title_text": "Generation of functional adipoids from human induced pluripotent stem cells",
  "type": "preprint",
  "updated_at": "2026-11-04T00:21:11Z",
  "url": "https://research.draconitic.com/paper/cwaj61bsj6zqh",
  "venue": "bioRxiv",
  "versions": []
}