{
  "abstract": "Background: Endometrial cancer is closely linked to obesity, insulin resistance and nutrition-related metabolic dysfunction. We examined lipid-synthesis and ferroptosis-related programs across tumor cell states and assessed lipogenic and ferroptosis-regulatory gene expression under hypoxia.\n\nMethods: Six single-cell RNA-sequencing libraries from three primary endometrial tumors and one Visium section were analyzed alongside 545 TCGA-UCEC tumors. Lipid-metabolism, ferroptosis, hypoxia and immune scores were evaluated at cellular, spatial and bulk-tissue levels. HEC-1-A and KLE cells were cultured under normoxia or hypoxia for 24 h, with three independent cultures per condition, and gene expression was assessed by qRT-PCR.\n\nResults: After quality control and doublet removal, 4,167 cells were retained and assigned to 14 annotated populations. Epithelial cells had the highest median lipid-synthesis score. Among 1,273 epithelial-lineage cells, hypoxia was weakly correlated with the ferroptosis-driver score ( r = 0.091, FDR = 0.0096), but not with defense. Across 1,347 spatial spots, epithelial and lipid-synthesis scores were positively correlated ( r = 0.191, FDR = 1.5 × 10–11). TCGA resistance scores were lower in serous than endometrioid tumors and higher in MSI-H than MSS tumors. Resistance was not independently associated with overall survival (HR = 0.83, 95% CI 0.47–1.47) and was unrelated to BMI. Under hypoxia, SREBF1, FASN and SCD expression was higher in both cell lines, together with CA9, GPX4, SLC7A11, AIFM2 and ACSL4.\n\nConclusion: Lipid-synthesis and ferroptosis-related programs varied across endometrial-cancer cell states and tissue regions. Hypoxic cell models showed concurrent changes in lipogenic and ferroptosis-regulatory transcripts. These findings identify metabolic transcriptional responses for functional investigation, without establishing a dietary effect or independent prognostic value for the resistance score.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Gynecology, Yiwu Central Hospital",
    "Department of Obstetrics and Gynaecology, Huai'an Hospital of Huai'an City (Huai'an Clinical Medical College of Jiangsu University)",
    "Department of Obstetrics and Gynaecology, Handan Central Hospital",
    "Department of Pathology, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xiaozhen Lou"
    },
    {
      "affs": [
        1
      ],
      "name": "Weiwei Cai"
    },
    {
      "affs": [
        2
      ],
      "name": "Jingjing Wang"
    },
    {
      "affs": [
        3
      ],
      "name": "Jinrong Wu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cx7fpntbxxmax.bib",
  "cited_by": 0,
  "doi": "10.3389/fpubh.2026.1991840",
  "fulltext_url": null,
  "id": "cx7fpntbxxmax",
  "issns": [
    "2296-2565"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fpubh.2026.1991840/full",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-04",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/cx7fpntbxxmax.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:21:48Z",
      "native_id": "10.3389/fpubh.2026.1991840",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fpubh.2026.1991840/full"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "issn:2296-2565",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Nutrition-related lipid metabolism and ferroptosis in endometrial cancer: transcriptomic profiling and hypoxia-associated gene expression",
  "title_text": "Nutrition-related lipid metabolism and ferroptosis in endometrial cancer: transcriptomic profiling and hypoxia-associated gene expression",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T00:21:28Z",
  "url": "https://research.draconitic.com/paper/cx7fpntbxxmax",
  "venue": "Frontiers in Public Health",
  "versions": []
}