{
  "abstract": "East Asian Passiflora virus (EAPV) is a significant viral pathogen prevalent across passionfruit cultivation regions in China and causes substantial economic losses to the passionfruit industry. The development of a sensitive, rapid, and accurate diagnostic method is essential for virus identification and epidemiological surveillance to support effective disease management strategies. In this study, a SYBR Green-based real-time quantitative PCR (qPCR) assay was developed for EAPV detection using a specific primer set targeting the viral coat protein (CP) gene. The assay was optimized with a primer concentration of 0.2 μmol/L and an annealing temperature of 60°C. The primers exhibited high specificity, generating a standard curve with an amplification efficiency of 90.9% and a coefficient of determination (R2) of 0.992. The limit of detection was 11.41 × 102 copies/μL, representing a 1000-fold greater sensitivity than conventional PCR. Moreover, viral accumulation was successfully detected in both inoculated and systemic leaves of passionfruit plants. In 2025, a total of 120 suspected virus-infected passionfruit samples were collected from Yunnan Province, China, and all samples tested positive for EAPV using the developed qPCR assay. Overall, the SYBR Green-based qPCR method established in this study demonstrated high specificity and sensitivity, providing a reliable tool for rapid EAPV diagnosis and epidemiological investigations.",
  "abstract_shown": true,
  "affiliations": [
    "Biotechnology and Genetic Germplasm Resources Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Kunming, China",
    "Forestry College/Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Faculty of Biodiversity Conservation, Southwest Forestry University, Kunming, China",
    "School of Ethnic Medicine, Yunnan Minzu University, Kunming, China",
    "Riphah International University, Faisalabad Campus, Faisalabad, Pakistan"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Hanye Zhou"
    },
    {
      "affs": [
        1
      ],
      "name": "Jie Zhang"
    },
    {
      "affs": [
        2
      ],
      "name": "Yu Li"
    },
    {
      "affs": [
        3
      ],
      "name": "Ziran Gao"
    },
    {
      "name": "Muhammad Qasim Aslam"
    },
    {
      "name": "Jin Xu"
    },
    {
      "name": "Yongdui Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cyy0g2269kwdj.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0356266",
  "fulltext_url": null,
  "id": "cyy0g2269kwdj",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0356266",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Virus Research Studies"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Virology/Viral replication"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Artificial gene amplification and extension/Polymerase chain reaction"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Artificial gene amplification and extension/Polymerase chain reaction/Reverse transcriptase-polymerase chain reaction"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Viruses/RNA viruses/Potyvirus"
    ],
    [
      "plos",
      "/Biology and life sciences/Plant science/Plant anatomy/Leaves"
    ],
    [
      "plos",
      "/Biology and life sciences/Plant science/Plant pathology/Plant pathogens/Plant viral pathogens"
    ],
    [
      "plos",
      "/Engineering and technology/Signal processing/Signal amplification"
    ],
    [
      "plos",
      "/Medicine and health sciences/Medical conditions/Infectious diseases/Viral diseases"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Artificial gene amplification and extension/Polymerase chain reaction"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Artificial gene amplification and extension/Polymerase chain reaction/Reverse transcriptase-polymerase chain reaction"
    ]
  ],
  "openalex": "W7211924790",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0356266&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-08",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/cyy0g2269kwdj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:56Z",
      "native_id": "10.1371/journal.pone.0356266",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0356266"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:molecular biology",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "plos:virology",
      "subfield": "microbiology"
    },
    {
      "field": "biology",
      "origin": "plos:plant science",
      "subfield": "plant-biology"
    }
  ],
  "title": "Establishment and application of a real-time fluorescence quantitative PCR assay for detecting EAPV in Passiflora edulis",
  "title_text": "Establishment and application of a real-time fluorescence quantitative PCR assay for detecting EAPV in Passiflora edulis",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:56Z",
  "url": "https://research.draconitic.com/paper/cyy0g2269kwdj",
  "venue": "PLOS One",
  "versions": []
}