{
  "abstract": "In this study, a molecularly imprinted polymer (MIP)-based electrochemical biosensor was developed for the selective detection of Escherichia coli (E. coli). The MIP recognition layer was synthesized by free-radical polymerization using MAM, AAM and VP as functional monomers with E. coli cells as the imprinting template. The MIP was immobilized onto a screen-printed electrode and evaluated using cyclic voltammetry (CV). Six MIP formulations with different MAM:AAM ratios were investigated to determine the optimal monomer composition. The optimized formulation, with an MAM:AAM ratio of 2:3:1, was evaluated over an E. coli concentration range of 10–105 CFU/mL and exhibited a linear response with a slope of 4.1107 and an R2 value of 0.9893 with a detection limit of 1.52 CFU/mL. The selectivity of the optimized sensor was further evaluated using Enterococcus faecalis and Pseudomonas aeruginosa, which showed lower responses than E. coli. These results indicate that the proposed MIP-based electrochemical biosensor provides preferential recognition of E. coli under the investigated conditions and demonstrates potential for bacterial detection.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical Engineering, School of Engineering, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand",
    "Department of Physics, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Chuchart Pintavirooj"
    },
    {
      "affs": [
        1
      ],
      "name": "Naphatsawan Vongmanee"
    },
    {
      "affs": [
        0
      ],
      "name": "Sarinporn Visitsattapongse",
      "orcid": "0009-0004-5890-6427"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cz0xgn1gryrnf.bib",
  "cited_by": 0,
  "doi": "10.3390/polym18202508",
  "fulltext_url": "https://europepmc.org/articles/PMC13708840",
  "id": "cz0xgn1gryrnf",
  "issns": [
    "2073-4360"
  ],
  "landing_url": "https://www.mdpi.com/2073-4360/18/20/2508",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1602|Analytical chemistry methods development"
    ]
  ],
  "openalex": "W7221748878",
  "pdf_url": "https://europepmc.org/articles/PMC13708840?pdf=render",
  "pmcid": "PMC13708840",
  "pmid": "42942629",
  "published": "2026-10-14",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/cz0xgn1gryrnf.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:44Z",
      "native_id": "10.3390/polym18202508",
      "source": "crossref",
      "url": "https://www.mdpi.com/2073-4360/18/20/2508"
    },
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42942629",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42942629"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1602|Analytical chemistry methods development",
      "subfield": "analytical"
    }
  ],
  "title": "Development and Optimization of a Molecularly Imprinted Polymer for Electrochemical Detection of Escherichia coli",
  "title_text": "Development and Optimization of a Molecularly Imprinted Polymer for Electrochemical Detection of Escherichia coli",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/cz0xgn1gryrnf",
  "venue": "Polymers",
  "versions": []
}