{
  "abstract": "Key message\n\nThe function of the phytochrome B photoreceptor is enhanced by its homologous counterpart, phytochrome D, at low temperatures, thereby increasing phenotypic plasticity in response to fluctuating environmental conditions. Light is a dynamically changing environmental factor that influences nearly every aspect of plant growth and development. Photoreceptors detect light, with phytochromes (PHY) specifically monitoring red and far-red wavelengths. Arabidopsis thaliana has five phytochromes, named phyA through phyE. phyA and phyB are extensively studied key mediators of most phytochrome responses; the precise functions of the other phytochromes remain poorly understood. To investigate phyD signaling, we overexpressed phyD-YFP alone and together with phyB-CFP in a genetic background lacking other phytochromes. Photomorphogenic responses, protein accumulation, and transcriptome changes were compared at low and high temperatures. We found that phyD-YFP alone cannot facilitate photomorphogenesis, but it significantly promotes red light signaling of both phyB-CFP and endogenous phyB in a temperature-dependent manner, being effective at low but not at high temperatures. In parallel, co-expressing phyD-YFP and phyB-CFP triggered substantially more robust light-induced transcriptome changes at low temperatures than expressing either transgene alone. Because phyD-YFP is more light-stable than phyB, and both PHYB-CFP and endogenous PHYB accumulate to higher levels alongside PHYD-YFP in light, phyD likely stimulates phyB signaling via PHYB protein stabilization. To explain how PHYD-YFP enhances PHYB levels at both temperatures yet stimulates signaling exclusively at low temperatures, we propose that phyD and phyB form light-stable heterodimers at both temperatures and that these heterodimers are functional only at low temperatures. Our findings demonstrate that phyB and phyD functionally cooperate to mediate temperature-dependent light signaling responses, providing plants with developmental flexibility under changing environmental conditions.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Photo and Chronobiology, Institute of Plant Biology, Biological Research Centre, Hungarian Research Network (HUN-REN), Szeged, 6726, Hungary.",
    "Faculty of Sciences and Informatics, Doctoral School of Biology, University of Szeged, Szeged, 6726, Hungary.",
    "Metabolic Systems Biology Laboratory, HCEMM-BRC, and Genomic Epidemiology Research Group, HUN-REN-BRC-ELTE-SZTE-NNGyK, Szeged, 6726, Hungary.",
    "Synthetic and Systems Biology Unit, Biological Research Centre, Institute of Biochemistry, Hungarian Research Network (HUN-REN), Szeged, 6726, Hungary.",
    "Laboratory of Photo and Chronobiology, Institute of Plant Biology, Biological Research Centre, Hungarian Research Network (HUN-REN), Szeged, 6726, Hungary. silhavy.daniel@brc.hu.",
    "Laboratory of Photo and Chronobiology, Institute of Plant Biology, Biological Research Centre, Hungarian Research Network (HUN-REN), Szeged, 6726, Hungary. viczian.andras@brc.hu."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Lilla Sípos"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Gábor Grézal"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Csaba Péter"
    },
    {
      "affs": [
        0
      ],
      "name": "Ferenc Nagy"
    },
    {
      "affs": [
        4
      ],
      "name": "Dániel Silhavy"
    },
    {
      "affs": [
        5
      ],
      "name": "András Viczián",
      "orcid": "0000-0003-2055-3430"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/cz9ctqkfvtkdg.bib",
  "cited_by": 0,
  "doi": "10.1007/s00299-026-04025-y",
  "fulltext_url": "https://europepmc.org/articles/PMC13694552",
  "id": "cz9ctqkfvtkdg",
  "issns": [
    "0721-7714",
    "1432-203X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42909509",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Light effects on plants"
    ]
  ],
  "openalex": "W7223301592",
  "pdf_url": "https://europepmc.org/articles/PMC13694552?pdf=render",
  "pmcid": "PMC13694552",
  "pmid": "42909509",
  "published": "2026-10-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/cz9ctqkfvtkdg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:03Z",
      "native_id": "MED:42909509",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42909509"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Light effects on plants",
      "subfield": "plant-biology"
    }
  ],
  "title": "Transgenic phytochrome D cooperates with phytochrome B to promote photomorphogenesis at low temperatures.",
  "title_text": "Transgenic phytochrome D cooperates with phytochrome B to promote photomorphogenesis at low temperatures.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:03Z",
  "url": "https://research.draconitic.com/paper/cz9ctqkfvtkdg",
  "venue": "Plant cell reports",
  "versions": []
}